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Literature summary for 3.3.2.11 extracted from
- 7-Dehydrocholesterol 5,6 beta-oxide as a mechanism-based inhibitor of microsomal cholesterol oxide hydrolase (1986), J. Biol. Chem., 261, 2510-2513.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | activates at 3% v/v | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,6alpha-imino-5alpha-cholestan-3beta-ol | strong, specific, competitive inhibition | Rattus norvegicus | |
| 7-dehydrocholesterol 5,6 beta-oxide | specific, mechanism-based inhibitor | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
| additional information | Rattus norvegicus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Long Evans rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially by microsome preparation | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O = 5alpha-cholestane-3beta,5alpha,6beta-triol | positively charged transition state | Rattus norvegicus | |
| 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O = 5alpha-cholestane-3beta,5alpha,6beta-triol | positively charged transition state | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cholesterol oxide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics at pH 8.0, 37°C, 6.5% v/v acetonitrile | Rattus norvegicus |
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