Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | activates, maximal at 6.25% v/v | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6alpha-imino-5alpha-cholestan-3beta-ol | strong inhibition | Rattus norvegicus | |
9,10-iminophenanthrene | - |
Rattus norvegicus | |
cholestane-3beta,5alpha,6alpha-triol | IC50 is 0.110 mM | Rattus norvegicus | |
cholestane-3beta,5alpha,6beta-triol | IC50 is 0.03 mM | Rattus norvegicus | |
cholestane-3beta,5beta,6alpha-triol | IC50 is 0.085 mM | Rattus norvegicus | |
cholestane-3beta,5beta,6beta-triol | IC50 is 0.118 mM | Rattus norvegicus | |
cholesterol | IC50 is above 0.125 mM | Rattus norvegicus | |
Iminosqualene | - |
Rattus norvegicus | |
oxidosqualene | IC50 is above 0.16 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Mus musculus | |
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | kinetics | Rattus norvegicus | |
additional information | - |
additional information | kinetics | Oryctolagus cuniculus | |
additional information | - |
additional information | kinetics | Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Mus musculus | - |
- |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Oryctolagus cuniculus | - |
- |
microsome | - |
Mesocricetus auratus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Mus musculus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Homo sapiens | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Oryctolagus cuniculus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Mesocricetus auratus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
additional information | Mus musculus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | ? | - |
? | |
additional information | Homo sapiens | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | ? | - |
? | |
additional information | Rattus norvegicus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | ? | - |
? | |
additional information | Oryctolagus cuniculus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | ? | - |
? | |
additional information | Mesocricetus auratus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mesocricetus auratus | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
liver microsome preparation | Mus musculus |
liver microsome preparation | Homo sapiens |
liver microsome preparation | Rattus norvegicus |
liver microsome preparation | Oryctolagus cuniculus |
liver microsome preparation | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
liver | - |
Mesocricetus auratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00004 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Oryctolagus cuniculus |
0.0001 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Mesocricetus auratus |
0.00014 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Oryctolagus cuniculus |
0.00018 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Mus musculus |
0.0002 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Mesocricetus auratus |
0.00038 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Homo sapiens |
0.00051 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Mus musculus |
0.00052 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
0.00108 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Homo sapiens |
0.00108 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Mus musculus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Homo sapiens | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Oryctolagus cuniculus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Mesocricetus auratus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Mus musculus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Homo sapiens | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Oryctolagus cuniculus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Mesocricetus auratus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Mus musculus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Homo sapiens | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Oryctolagus cuniculus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Mesocricetus auratus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | Mus musculus | ? | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | Homo sapiens | ? | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | Rattus norvegicus | ? | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | Oryctolagus cuniculus | ? | - |
? | |
additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9, which shows a wider substrate specificity | Mesocricetus auratus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Oryctolagus cuniculus |
37 | - |
assay at | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |
7.4 | - |
assay at | Rattus norvegicus |
7.4 | - |
assay at | Oryctolagus cuniculus |
7.4 | - |
assay at | Mesocricetus auratus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
5,6alpha-imino-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
0.0366 | - |
Iminosqualene | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
0.126 | - |
9,10-iminophenanthrene | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.03 | - |
IC50 is 0.03 mM | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | |
0.085 | - |
IC50 is 0.085 mM | Rattus norvegicus | cholestane-3beta,5beta,6alpha-triol | |
0.11 | - |
IC50 is 0.110 mM | Rattus norvegicus | cholestane-3beta,5alpha,6alpha-triol | |
0.118 | - |
IC50 is 0.118 mM | Rattus norvegicus | cholestane-3beta,5beta,6beta-triol | |
0.125 | - |
IC50 is above 0.125 mM | Rattus norvegicus | cholesterol | |
0.16 | - |
IC50 is above 0.16 mM | Rattus norvegicus | oxidosqualene |