Cloned (Comment) | Organism |
---|---|
gene EPHX2, expression of His-tagged wild-type and mutant enzymes in Spodoptera frugiperda Sf21 cells using the baculovirus infection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C154Y | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 2.2, compared to the wild-type ratio of 1.0 | Homo sapiens |
E470G | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 0.9, compared to the wild-type ratio of 1.0 | Homo sapiens |
K55R | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.9, compared to the wild-type ratio of 1.0 | Homo sapiens |
R103C | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.3, compared to the wild-type ratio of 1.0 | Homo sapiens |
R103C/R287Q | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 1.2, compared to the wild-type ratio of 1.0 | Homo sapiens |
R287Q | site-directed mutagenesis, the mutant shows the same hydrolase/phosphatase ratio of 1.0 as the wild-type, but reduced stability at 37°C | Homo sapiens |
V422A | site-directed mutagenesis, the mutant shows a hydrolase/phosphatase ratio of 0.8, compared to the wild-type ratio of 1.0 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene EPHX2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Sf21 insect cells by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-stilbene oxide + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal part harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC 3.1.3.76 is located at the N-terminal part, Arg287 may be involved in formation of the quaternary enzyme structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | see also EC 3.1.3.76 | Homo sapiens |
SEH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
inactivation rate constants for recombinant wild-type and mutant enzymes | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |