Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a target for regulation of blood pressure, and inhibition of inflammation, atherosclerosis, kidney failure, and cancer progression | Homo sapiens |
pharmacology | the enzyme is a target for inhibitor design | Mus musculus |
pharmacology | the enzyme is a target for inhibitor design | Homo sapiens |
pharmacology | the enzyme is a target for inhibitor design | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
enzyme with bound inhibitor N-cyclohexyl-N'-decylurea | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H431S | mutation of His431 results in total loss of activity, the mutant can form the intermediate but cannot hydrolyze it | Mus musculus |
Y381F | site-directed mutagenesis, mutant shows 90% reduced activity | Mus musculus |
Y465F | site-directed mutagenesis, mutant shows 90% reduced activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-disubstituted amides | potent and stable inhibition, Ki in the nanomolar range, mechanism | Homo sapiens | |
1,3-disubstituted amides | potent and stable inhibition, Ki in the nanomolar range, mechanism | Mus musculus | |
1,3-disubstituted amides | potent and stable inhibition, Ki in the nanomolar range, mechanism | Rattus norvegicus | |
1,3-disubstituted carbamate derivatives | potent and stable inhibition, Ki in the nanomolar range, mechanism | Homo sapiens | |
1,3-disubstituted carbamate derivatives | potent and stable inhibition, Ki in the nanomolar range, mechanism | Mus musculus | |
1,3-disubstituted carbamate derivatives | potent and stable inhibition, Ki in the nanomolar range, mechanism | Rattus norvegicus | |
1,3-disubstituted urea derivatives | potent and stable inhibition, Ki in the nanomolar range, mechanism | Homo sapiens | |
1,3-disubstituted urea derivatives | potent and stable inhibition, Ki in the nanomolar range, mechanism | Mus musculus | |
1,3-disubstituted urea derivatives | potent and stable inhibition, Ki in the nanomolar range, mechanism | Rattus norvegicus | |
Cd2+ | - |
Homo sapiens | |
Cd2+ | - |
Mus musculus | |
Cd2+ | - |
Rattus norvegicus | |
Cu2+ | - |
Homo sapiens | |
Cu2+ | - |
Mus musculus | |
Cu2+ | - |
Rattus norvegicus | |
Hg2+ | - |
Homo sapiens | |
Hg2+ | - |
Mus musculus | |
Hg2+ | - |
Rattus norvegicus | |
additional information | most enzyme inhibitors do not influence the enzyme's phosphatase activity | Homo sapiens | |
N-cyclohexyl-N'-decylurea | - |
Mus musculus | |
Zn2+ | noncompetitive, inhibits epoxide hydrolase and phosphatase activities, allosteric mechanism | Homo sapiens | |
Zn2+ | - |
Mus musculus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, the hydrolysis of the intermediate is the rate-limiting step | Mus musculus | |
additional information | - |
additional information | kinetics, the hydrolysis of the intermediate is the rate-limiting step | Homo sapiens | |
additional information | - |
additional information | kinetics, the hydrolysis of the intermediate is the rate-limiting step | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62500 | - |
1 * 62500 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the enzyme is involved in regulation of blood pressure and inflammation | ? | - |
? | |
additional information | Homo sapiens | the enzyme is involved in regulation of blood pressure and inflammation | ? | - |
? | |
additional information | Rattus norvegicus | the enzyme is involved in regulation of blood pressure and inflammation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an epoxide + H2O = a glycol | two step mechanism with formation of of a hydroxyl-alkyl-enzyme intermediate, catalytic triad is Asp333-His523-Asp495 with polarizing residues Tyr381 and Tyr465, catalytic cycle | Mus musculus | |
an epoxide + H2O = a glycol | two step mechanism with formation of of a hydroxyl-alkyl-enzyme intermediate, the catalytic triad is Asp333-His523-Asp495, catalytic cycle | Rattus norvegicus | |
an epoxide + H2O = a glycol | two step mechanism with formation of of a hydroxyl-alkyl-enzyme intermediate, the catalytic triad is Asp334-His523-Asp495 with polarizing residues Tyr382 and Tyr465, catalytic cycle | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
kidney | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
additional information | wide tissue distribution | Mus musculus | - |
additional information | wide tissue distribution | Homo sapiens | - |
additional information | wide tissue distribution | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chalcone oxides + H2O | - |
Mus musculus | ? | - |
? | |
chalcone oxides + H2O | - |
Homo sapiens | ? | - |
? | |
chalcone oxides + H2O | - |
Rattus norvegicus | ? | - |
? | |
additional information | the enzyme is involved in regulation of blood pressure and inflammation | Mus musculus | ? | - |
? | |
additional information | the enzyme is involved in regulation of blood pressure and inflammation | Homo sapiens | ? | - |
? | |
additional information | the enzyme is involved in regulation of blood pressure and inflammation | Rattus norvegicus | ? | - |
? | |
additional information | sEH prefers gem-di-, trans-di-, cis-di-, tri-, and tetra-substituted epoxides | Mus musculus | ? | - |
? | |
additional information | sEH prefers gem-di-, trans-di-, cis-di-, tri-, and tetra-substituted epoxides | Rattus norvegicus | ? | - |
? | |
additional information | the enzyme also shows phosphatase activity, EC 3.1.3.76, sEH prefers gem-di-, trans-di-, cis-di-, tri-, and tetra-substituted epoxides | Homo sapiens | ? | - |
? | |
stearic acid epoxide + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62500 | Homo sapiens |
More | quarternary structure | Mus musculus |
More | quarternary structure | Rattus norvegicus |
More | quarternary structure, monomers interact in a head-to-tail manner, the enzyme contains the DXDX(T/V) motif | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
EPXH1 | - |
Homo sapiens |
EPXH2 | - |
Homo sapiens |
More | the enzyme belongs to the alpha/beta-hydrolase fold family of proteins | Mus musculus |
More | the enzyme belongs to the alpha/beta-hydrolase fold family of proteins | Homo sapiens |
More | the enzyme belongs to the alpha/beta-hydrolase fold family of proteins | Rattus norvegicus |
SEH | - |
Mus musculus |
SEH | - |
Homo sapiens |
SEH | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Zn2+ | - |
Homo sapiens |