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Literature summary for 3.3.2.10 extracted from

  • Bellevik, S.; Summerer, S.; Meijer, J.
    Overexpression of Arabidopsis thaliana soluble epoxide hydrolase 1 in Pichia pastoris and characterisation of the recombinant enzyme (2002), Protein Expr. Purif., 26, 65-70.
    View publication on PubMed
Search for more literature for 3.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Pichia pastoris. A tag containing six His residues is added to the N-terminus to enable efficient one-step purification Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 trans-stilbene oxide pH 7.0, 30°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Arabidopsis thaliana
38061
-
 x * 38061, MALDI-TOF Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Storage Stability

Storage Stability Organism
4°C, 6 month, stable Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-stilbene oxide + H2O activity is 50fold lower than with trans-stilbene oxide Arabidopsis thaliana 1,2-diphenylethane-1,2-diol
-
 ?
trans-stilbene oxide + H2O
-
 Arabidopsis thaliana 1,2-diphenylethane-1,2-diol
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Arabidopsis thaliana
? x * 38061, MALDI-TOF Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
epoxide hydrolase 1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
54
-
 activity towards trans-stilbene oxide Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
21
-
 4 h, 50% loss of activity Arabidopsis thaliana
30
-
 3.5 h, 50% loss of activity Arabidopsis thaliana
37
-
 2.8 h, 50% loss of activity Arabidopsis thaliana
45
-
 2.1 h, 50% loss of activity Arabidopsis thaliana
54
-
 1 h, 50% loss of activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 trans-stilbene oxide pH 7.0, 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity towards trans-stilbene oxide Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 pH 4.5: about 90% of maximal activity, pH 5.0: about 50% of maximal activity, 6.0: about 60% of maximal activity, pH 8.5: about 90% of maximal acticity, pH 9.0: about 70% of maximal activity, pH 10.0: about 35% of maximal activity Arabidopsis thaliana