Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in insect cells using the baculovirus infection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y465F | site-directed mutagenesis, mutant enzyme shows phosphatase, but no epoxide hydrolase activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-dicyclohexyl urea | 0.1 mM, complete inhibition of epoxide hydrolase activity, no inhibition of phosphatase activity; complete inhibition at 0.1 mM | Homo sapiens | |
1-cyclohexyl-3-dodecyl urea | 0.1 mM, complete inhibition of epoxide hydrolase activity, no inhibition of phosphatase activity; complete inhibition at 0.1 mM | Homo sapiens | |
1-cyclohexyl-3-ethyl urea | 0.1 mM, 54% inhibition of epoxide hydrolase activity, no inhibition of phosphatase activity; 54% inhibition at 0.1 mM | Homo sapiens | |
1-cyclohexyl-3-hexyl urea | 0.1 mM, complete inhibition of epoxide hydrolase activity, 4% inhibition of phosphatase activity; complete inhibition at 0.1 mM | Homo sapiens | |
4-fluoro-chalcone oxide | 0.1 mM, 87% inhibition of epoxide hydrolase activity, 8% inhibition of phosphatase activity | Homo sapiens | |
4-fluorochalcone oxide | 87% inhibition at 0.1 mM, competitive substrate | Homo sapiens | |
4-phenyl-chalcone oxide | 0.1 mM, complete inhibition of epoxide hydrolase activity, 11% inhibition of phosphatase activity | Homo sapiens | |
4-Phenylchalcone oxide | complete inhibition at 0.1 mM, competitive substrate | Homo sapiens | |
Ca2+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
Cd2+ | inhibition of phosphatase activity and epoxide hydrolase activity | Homo sapiens | |
Ce3+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
Chalcone oxide | 0.1 mM, 80% inhibition of epoxide hydrolase activity, no inhibition of phosphatase activity; 80% inhibition at 0.1 mM, competitive substrate | Homo sapiens | |
Co2+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
Cu+ | inhibition of phosphatase activity and epoxide hydrolase activity | Homo sapiens | |
Cu2+ | inhibition of phosphatase activity and epoxide hydrolase activity | Homo sapiens | |
Fe2+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
Fe3+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
Hg2+ | inhibition of phosphatase activity and epoxide hydrolase activity | Homo sapiens | |
Mn2+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
additional information | poor or no inhibition by tartaric acid, sodium fluoride, and sodium molybdate | Homo sapiens | |
okadaic acid | 12% inhibition at 0.1 mM | Homo sapiens | |
Pb2+ | inhibition of Mg2+-dependent acrtivity | Homo sapiens | |
sodium orthovanadate | 9% inhibition at 0.1 mM | Homo sapiens | |
Zn2+ | inhibition of phosphatase activity and epoxide hydrolase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0209 | - |
threo-10-hydroxy-9-phosphonooxy-octadecanoic acid | - |
Homo sapiens | |
1.6 | - |
p-nitrophenyl phosphate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ce3+ | enhances activity | Homo sapiens | |
Co2+ | 50% of the activation with Mg2+ | Homo sapiens | |
Mg2+ | enhances activity | Homo sapiens | |
Ni2+ | enhances activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | - |
gene EPXH2 | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
recombinant wild-type and mutant enzymes from insect cells by affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an epoxide + H2O = a glycol | the C-terminal part harbors the epoxide hydrolase activity, the phosphatase activity of the enzyme is located at the N-terminal part of EC 3.1.3.76, both catalytic sites act independently | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0064 | - |
epoxide hydrolysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-phosphonooxyoctadec-9E-enoic acid + H2O | - |
Homo sapiens | (9E)-octadecenoic acid + phosphate | - |
? | |
12-phosphonooxyoctadec-9Z-enoic acid + H2O | - |
Homo sapiens | (9Z)-octadecenoic acid + phosphate | - |
? | |
12-phosphonooxyoctadecanoic acid + H2O | - |
Homo sapiens | octadecanoic acid + phosphate | - |
? | |
erythro-10-hydroxy-9-phosphonooxy-octadecanoic acid + H2O | - |
Homo sapiens | 10-hydroxy-octadecanoic acid + phosphate | - |
? | |
additional information | the N-terminal domain of the enzyme is a functional phosphatase unaffected by a number of classic phosphatase inhibitors. The phosphatase domain has high specificity for lipophilic phosphates | Homo sapiens | ? | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Homo sapiens | ? | - |
? | |
threo-10-hydroxy-9-phosphonooxy-octadecanoic acid + H2O | - |
Homo sapiens | 10-hydroxy-octadecanoic acid + phosphate | - |
? | |
trans-diphenyl propene oxide + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the 25 kDa C-terminal part, with a hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity, the enzyme's phosphatase activity of EC3.1.3.76 is located at the 35 kDa N-terminal part which has a different alpha/beta-fold, structure analysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
EPXH2 | - |
Homo sapiens |
More | see also EC 3.1.3.76 | Homo sapiens |
SEH | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
p-nitrophenyl phosphate | - |
Homo sapiens | |
0.35 | - |
threo-10-hydroxy-9-phosphonooxy-octadecanoic acid | - |
Homo sapiens |