Application | Comment | Organism |
---|---|---|
agriculture | the isolation of BNSEH1 will facilitate metabolic studies of resistance and seed oil modification in the important oilcrop Brassica napus | Brassica napus |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Brassica napus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-naphthoflavone | 1 mM, 50% inhibition | Brassica napus | |
Chalcone oxide | 1 mM, 98% inhibition | Brassica napus | |
Dimethylsulfoxide | - |
Brassica napus | |
N,N'-dicyclohexylcarbodiimide | 0.01 mM, complete inhibition | Brassica napus | |
N,N'-dicyclohexylurea | 0.01 mM, 30% inhibition | Brassica napus | |
p-hydroxymercuribenzoate | - |
Brassica napus | |
quercetin | 1 mM, 75% inhibition | Brassica napus | |
tetrahydrofuran | - |
Brassica napus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
trans-stilbene oxide | pH 7.0, 30°C | Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Brassica napus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 37000, SDS-PAGE | Brassica napus |
45000 | - |
gel filtration | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-stilbene oxide + H2O | low activity | Brassica napus | 1,2-diphenylethane-1,2-diol | - |
? | |
trans-stilbene oxide + H2O | - |
Brassica napus | 1,2-diphenylethane-1,2-diol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Brassica napus |
Synonyms | Comment | Organism |
---|---|---|
BNSEH1 | - |
Brassica napus |
SEH | - |
Brassica napus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
reaction with trans-stilbene oxide | Brassica napus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 35% of maximal activity, 55°C: maximum, 65°C: no activity, reaction with trans-stilbene oxide | Brassica napus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
trans-stilbene oxide | pH 7.0, 30°C | Brassica napus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | reaction with trans-stilbene oxide | Brassica napus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, reaction with trans-stilbene oxide | Brassica napus |