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Literature summary for 3.3.2.10 extracted from

  • Schladt, L.; Thomas, H.; Hartmann, R.; Oesch, F.
    Human liver cytosolic epoxide hydrolases (1988), Eur. J. Biochem., 176, 715-723.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
7,8-styrene oxide
-
Homo sapiens
trichloropropene oxide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
cis-2-methylstyrene oxide cytosolic enzyme Homo sapiens
0.083
-
cis-1,2-dimethylstyrene oxide microsomal enzyme Homo sapiens
0.089
-
cis-1,2-dimethylstyrene oxide cytosolic enzyme Homo sapiens
0.143
-
7,8-styrene oxide microsomal enzyme Homo sapiens
0.229
-
2,2-dimethylstyrene oxide microsomal enzyme Homo sapiens
0.319
-
cis-2-methylstyrene oxide microsomal enzyme Homo sapiens
0.358
-
2,2-dimethylstyrene oxide cytosolic enzyme Homo sapiens
0.473
-
trans-1,2-dimethylstyrene oxide microsomal enzyme Homo sapiens
0.517
-
7,8-styrene oxide cytosolic enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
microsome
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.145
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-dimethylstyrene oxide + H2O
-
Homo sapiens ?
-
?
cis-1,2-dimethylstyrene oxide + H2O
-
Homo sapiens ?
-
?
cis-2-methylstyrene oxide + H2O
-
Homo sapiens ?
-
?
additional information microsomal enzyme shows highest activity with trans-2-methylstyrane oxide, followed by styrene 7,8-oxide, cis-2-methylstyrene oxide, cis-1,2-dimethylstyrene oxide, trans-1,2-dimethylstyrene oxide and 2,2-dimethylstyrene oxide. With the cytosolic enzyme the same order is obtained for the first three substrates, whereas activity with 2,2-dimethylstyrene oxide is higher than with cis-1,2-dimethylstyrene oxide and no hydration occurs with trans-1,2-dimethylstyrene oxide Homo sapiens ?
-
?
styrene 7,8-oxide + H2O
-
Homo sapiens styrene glycol
-
?
trans-1,2-dimethylstyrene oxide + H2O hydration by microsomal enzyme, no activity with cytosolic enzyme Homo sapiens ?
-
?
trans-2-methylstyrene oxide + H2O
-
Homo sapiens ?
-
?

pH Range

pH Minimum pH Maximum Comment Organism
7.4 9 cytosolic enzyme shows higher activity at pH 7.4, microsomal enzyme shows higher activity at pH 9.0 Homo sapiens