Literature summary for 3.3.2.1 extracted from

Kunzler, D.E.; Sasso, S.; Gamper, M.; Hilvert, D.; Kast, P.
Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection (2005), J. Biol. Chem., 280, 32827-32834.

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Protein Variants

Protein Variants	Comment	Organism
Q91N	4fold reduction in kcat-value of enzymic activity, 10fold reduction in chorismate mutase activity	Pseudomonas aeruginosa
R54K	1000fold reduction in enzymic and in chorismate mutase activity	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	isochorismate	mutant R54K, pH 7.5, 30°C	Pseudomonas aeruginosa
0.27	-	isochorismate	mutant Q91N, pH 7.5, 30°C	Pseudomonas aeruginosa
1.06	-	isochorismate	wild-type, pH 7.5, 30°C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	isoform PchB, possessing mainly chorismate lyase activity	-

Reaction

Reaction	Comment	Organism	Reaction ID
isochorismate + H2O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate	(1,5)-sigmatropic reaction mechanism that invokes electrostatic catalysis	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Chorismate	promiscuous reaction	Pseudomonas aeruginosa	Prephenate	-	?
isochorismate + H2O	main reaction	Pseudomonas aeruginosa	2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate	-	?

Synonyms

Synonyms	Comment	Organism
IPL	-	Pseudomonas aeruginosa
PchB	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.003	-	chorismate	mutant R54K, pH 7.5, 30°C	Pseudomonas aeruginosa
0.0062	-	chorismate	mutant Q91N, pH 7.5, 30°C	Pseudomonas aeruginosa
0.085	-	chorismate	wild-type, pH 7.5, 30°C	Pseudomonas aeruginosa
0.1	-	isochorismate	mutant R54K, pH 7.5, 30°C	Pseudomonas aeruginosa
0.27	-	isochorismate	mutant Q91N, pH 7.5, 30°C	Pseudomonas aeruginosa
1.06	-	isochorismate	wild-type, pH 7.5, 30°C	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)