Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) RIL cells | Arabidopsis thaliana |
isozyme AtMTAN2, DNA and amino acid sequence determination and analysis, overexpression of isozyme AtMTAN2 in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5) and 26-32% (w/v) PEG MME 2000 | Arabidopsis thaliana |
isozyme AtMTAN2, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modelling | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2009 | - |
S-adenosyl-L-homocysteine | in 100 mM sodium phosphate buffer, at pH 8.0 and 37°C | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + H2O | Arabidopsis thaliana | isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not | adenine + 5-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | Arabidopsis thaliana | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozyme AtMTAN2 | - |
Arabidopsis thaliana | Q7XA67 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione column chromatography, HiTrap Q Sepharose column chromatography, and Superose 12 gel filtration | Arabidopsis thaliana |
recombinant isozyme AtMTAN2 from Escherichia coli to homogeneity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificities of isozymes, both isozymes are active with S-methyl-5'-thioadenosine, EC 3.2.2.16, overview | Arabidopsis thaliana | ? | - |
? | |
S-adenosyl-L-homocysteine + H2O | isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not | Arabidopsis thaliana | adenine + 5-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Arabidopsis thaliana | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure of isozyme AtMTAN2, structure comparison e.g. of a flexible loop, overview. Active site structures and structure modelling, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine nucleosidase | - |
Arabidopsis thaliana |
5'-methylthioadenosine nucleosidases | - |
Arabidopsis thaliana |
MTAN | - |
Arabidopsis thaliana |
MTAN2 | - |
Arabidopsis thaliana |