Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Codon+ cells | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
5'-methylthioadenosine | in 100 mM sodium phosphate buffer, pH 7.0 | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27462 | - |
x * 27462, isoform MATN2, calculated from amino acid sequence | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-homocysteine + H2O | Arabidopsis thaliana | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9T0I8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
using S-adenosyl-L-homocysteine as substrate, at pH 6.0 and 22°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-butylthioadenosine + H2O | 24% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-butylthio-D-ribose | - |
? | |
5'-chloroadenosine + H2O | 13% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-chloro-D-ribose | - |
? | |
5'-ethylthioadenosine + H2O | 42% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-ethylthio-D-ribose | - |
? | |
5'-iodoadenosine + H2O | 17% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-iodo-D-ribose | - |
? | |
5'-isobutylthioadenosine + H2O | 32% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-isobutylthio-D-ribose | - |
? | |
5'-methylthioadenosine + H2O | 100% activity | Arabidopsis thaliana | 5-methylthio-D-ribose + adenine | - |
? | |
5'-phenylthioadenosine + H2O | 39% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + 5-phenylthio-D-ribose | - |
? | |
adenosine + H2O | 6% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + D-ribose | - |
? | |
S-adenosyl-L-homocysteine + H2O | 14% activity compared to 5'-methylthioadenosine | Arabidopsis thaliana | adenine + S-ribosyl-L-homocysteine | - |
? | |
S-adenosyl-L-homocysteine + H2O | - |
Arabidopsis thaliana | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27462, isoform MATN2, calculated from amino acid sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Arabidopsis thaliana |
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2 | - |
Arabidopsis thaliana |
MTA/SAH nucleosidase | - |
Arabidopsis thaliana |
MTAN2 | - |
Arabidopsis thaliana |
MTAN2 | isoform | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
5'-methylthioadenosine | in 100 mM sodium phosphate buffer, pH 7.0 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Arabidopsis thaliana |