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Literature summary for 3.2.2.9 extracted from

  • Lee, J.E.; Luong, W.; Huang, D.J.; Cornell, K.A.; Riscoe, M.K.; Howell, P.L.
    Mutational analysis of a nucleosidase involved in quorum-sensing autoinducer-2 biosynthesis (2005), Biochemistry, 44, 11049-11057.
    View publication on PubMed
Search for more literature for 3.2.2.9

Protein Variants

Protein Variants Comment Organism
D197A enzyme is inactive with 5'-methylthioadenosine and S-adenosyl-L-homocysteine Escherichia coli
D197N enzyme is inactive with 5'-methylthioadenosine and S-adenosyl-L-homocysteine Escherichia coli
E12A enzyme is inactive with 5'-methylthioadenosine and S-adenosyl-L-homocysteine Escherichia coli
E12Q enzyme is inactive with 5'-methylthioadenosine and S-adenosyl-L-homocysteine Escherichia coli
E174A enzyme is inactive with 5'-methylthioadenosine and S-adenosyl-L-homocysteine Escherichia coli
E174Q enzyme is inactive with 5'-methylthioadenosine and S-adenosyl-L-homocysteine Escherichia coli
F105A kcat/Km for 5'-methylthioadenosine is 87.4% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 128% of wild-type value Escherichia coli
F151A kcat/Km for 5'-methylthioadenosine is 0.5% of wild-type value kcat/Km for S-adenosyl-L-homocysteine is 0.2% of wild-type value Escherichia coli
F207A kcat/Km for 5'-methylthioadenosine is 2.2% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 2.7% of wild-type value Escherichia coli
I50A kcat/Km for 5'-methylthioadenosine is 11.8% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 23.3% of wild-type value Escherichia coli
M173A kcat/Km for 5'-methylthioadenosine is 0.6% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 0.1% of wild-type value Escherichia coli
M9A kcat/Km for 5'-methylthioadenosine is 12.6% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 18.6% of wild-type value Escherichia coli
R193A kcat/Km for 5'-methylthioadenosine is 12.9% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 28.1% of wild-type value Escherichia coli
S196A kcat/Km for 5'-methylthioadenosine is 2.3% of wild-type value kcat/Km for S-adenosyl-L-homocysteine is 4% of wild-type value Escherichia coli
S76A kcat/Km for 5'-methylthioadenosine is 13.4% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 22.9% of wild-type value Escherichia coli
V102A kcat/Km for 5'-methylthioadenosine is 95% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 233% of wild-type value Escherichia coli
Y107F kcat/Km for 5'-methylthioadenosine is 32.2% of wild-type value, kcat/Km for S-adenosyl-L-homocysteine is 58.1% of wild-type value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 5'-methylthioadenosine 21°C, pH 7.0, wild-type enzyme Escherichia coli
0.001
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme M9A Escherichia coli
0.0013
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, wild-type enzyme Escherichia coli
0.0014
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme M9A Escherichia coli
0.0017
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme F207A Escherichia coli
0.0017
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme S196A Escherichia coli
0.0017
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme Y107F Escherichia coli
0.0019
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme S76A Escherichia coli
0.0019
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme V102A Escherichia coli
0.002
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme R193A Escherichia coli
0.0022
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme Y107F Escherichia coli
0.0027
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme S76A Escherichia coli
0.0028
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F105A Escherichia coli
0.0029
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme S196A Escherichia coli
0.003
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme V102A Escherichia coli
0.0032
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme R193A Escherichia coli
0.0033
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme F105A Escherichia coli
0.0037
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme I50A Escherichia coli
0.0055
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F207A Escherichia coli
0.0071
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme I50A Escherichia coli
0.022
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme F151A Escherichia coli
0.034
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme M173A Escherichia coli
0.037
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F151A Escherichia coli
0.49
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme M173A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AF12
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
66
-
 Tm-value for mutant enzyme S196A and F105A Escherichia coli
67
-
 Tm-value for mutant enzymeV102A and Y107F Escherichia coli
68
-
 Tm-value for wild-type enzyme and mutant enzyme F151A Escherichia coli
70
-
 Tm-value for mutant enzymeS76A Escherichia coli
71
-
 Tm-value for mutant enzyme M173A and M9A Escherichia coli
72
-
 Tm-value for mutant enzyme R193A and F207A Escherichia coli
75
-
 Tm-value for mutant enzyme I50A Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme M9A Escherichia coli
0.0017
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme Y107F Escherichia coli
0.0019
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme S76A Escherichia coli
0.0019
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme V102A Escherichia coli
0.002
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme R193A Escherichia coli
0.0028
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F105A Escherichia coli
0.0037
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme I50A Escherichia coli
0.0055
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F207A Escherichia coli
0.13
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme F207A Escherichia coli
0.14
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F151A Escherichia coli
0.14
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme S196A Escherichia coli
0.23
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme S196A Escherichia coli
0.31
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F207A Escherichia coli
0.37
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme F151A Escherichia coli
0.37
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme M9A Escherichia coli
0.49
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme M173A Escherichia coli
0.63
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme M9A Escherichia coli
0.72
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme M173A Escherichia coli
0.8
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme M173A Escherichia coli
0.93
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme S76A Escherichia coli
1.2
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme R193A Escherichia coli
1.3
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme S76A Escherichia coli
1.5
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme R193A Escherichia coli
1.8
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme I50A Escherichia coli
2
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme Y107F Escherichia coli
2.5
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme Y107F Escherichia coli
2.6
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, wild-type enzyme Escherichia coli
3
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme I50A Escherichia coli
3
-
 5'-methylthioadenosine 21°C, pH 7.0, wild-type enzyme Escherichia coli
7.5
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme F105A Escherichia coli
9.1
-
 S-adenosyl-L-homocysteine 21°C, pH 7.0, mutant enzyme V102A Escherichia coli
10.2
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme F105A Escherichia coli
10.2
-
 5'-methylthioadenosine 21°C, pH 7.0, mutant enzyme V102A Escherichia coli