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Literature summary for 3.2.2.9 extracted from
- Cloning and expression of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: Identification of the pfs gene product (1998), Biochim. Biophys. Acta, 1396, 8-14.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels | Escherichia coli |
| medicine | potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels | Klebsiella pneumoniae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pfs gene cloned and expressed in pCRII using PCR, subcloned into pAL781 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenine | recombinant MTA/SAH'ase (rMTAN) | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00045 | - |
5'-methylthioadenosine | recombinant nucleosidase | Escherichia coli | |
| 0.00053 | - |
5'-methylthioadenosine | native nucleosidase | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24350 | - |
estimation from pfs gene product | Escherichia coli |
| 25000 | 31000 | native MTA/SAH'ase | Escherichia coli |
| 26000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | Escherichia coli | - |
? | - |
? | |
| 5'-methylthioadenosine + H2O | Klebsiella pneumoniae | - |
? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | Escherichia coli | - |
? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | Klebsiella pneumoniae | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-methylthioadenosine + H2O | - |
Escherichia coli | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Klebsiella pneumoniae | 5-methylthio-D-ribose + adenine | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Escherichia coli | ? | - |
? | |
| 5'-methylthioadenosine + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | adenine + S-ribosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Klebsiella pneumoniae | adenine + S-ribosyl-L-homocysteine | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Escherichia coli | ? | - |
? | |
| S-adenosyl-L-homocysteine + H2O | - |
Klebsiella pneumoniae | ? | - |
? | |
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