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Literature summary for 3.2.2.9 extracted from

  • Cornell, K.A.; Winter, R.W.; Tower, P.A.; Riscoe, M.K.
    Affinity purification of 5-methylthioadenosine kinase and 5-methylthioribose/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae (1996), Biochem. J., 317, 285-290.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.9

Application

Application Comment Organism
medicine potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels Escherichia coli
medicine potential target for chemotherapeutic intervention, nucleoside analogues which inhibit the nucleosidase could kill invading microorganisms by perturbing methylation processes and polyamine levels Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 5'-methylthioadenosine
-
 Escherichia coli
0.0004
-
 5'-methylthioadenosine
-
 Lupinus luteus
0.0087
-
 5'-methylthioadenosine
-
 Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 estimation from pfs gene product Klebsiella pneumoniae
26500
-
 SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
5'-methylthioadenosine + H2O Klebsiella aerogenes
-
 ?
-
 ?
5'-methylthioadenosine + H2O Klebsiella pneumoniae
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Escherichia coli
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Klebsiella aerogenes
-
 ?
-
 ?
S-adenosyl-L-homocysteine + H2O Klebsiella pneumoniae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Klebsiella aerogenes
-
-
-
Klebsiella pneumoniae
-
 gram-negative pathogen, clinical isolate
-
Lupinus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Lupinus luteus 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Klebsiella pneumoniae 5-methylthio-D-ribose + adenine
-
 ?
5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes ?
-
 ?
5'-methylthioadenosine + H2O
-
 Klebsiella pneumoniae ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Klebsiella pneumoniae adenine + S-ribosyl-L-homocysteine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes ?
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Klebsiella pneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26500, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
K. pneumoniae MTA/SAH nucleosidase
-
 Klebsiella pneumoniae