Cloned (Comment) | Organism |
---|---|
gene yeiK, subcloning in strain DH5alpha, expression of the His6-tagged enzyme in strain BL21(DE3) | Escherichia coli K-12 |
Crystallization (Comment) | Organism |
---|---|
purified detagged recombinant enzyme, hanging drop vapour diffusion method, 10 mg/ml protein in 20 mM HEPES, pH 7.4, 150 mM NaCl, is mixed with precipitant solution, 25°C, equilibration versus reservoir solution containing 100 mM Tris-HCl, pH 8.5, 200 mM NaCl, 25% w/v PEG 3350, cryoprotection by 25% glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, detagged recombinant enzyme, SDS-PAGE | Escherichia coli K-12 |
143000 | - |
detagged recombinant enzyme, gel filtration | Escherichia coli K-12 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
gene yeiK | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from strain BL21(DE3) by nickel chelate affinity chromatography and gel filtration, the His-tag is cleaved off by thrombin | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouridine + H2O | detagged recombinant enzyme | Escherichia coli K-12 | 5-fluorouracil + D-ribose | - |
? | |
cytidine + H2O | detagged recombinant enzyme | Escherichia coli K-12 | cytosine + D-ribose | - |
? | |
additional information | detagged recombinant enzyme: substrate specificity, no activity with purine nucleosides, and with 2'-, 3'-, and 5'-deoxynucleosides | Escherichia coli K-12 | ? | - |
? | |
uridine + H2O | detagged recombinant enzyme | Escherichia coli K-12 | uracil + D-ribose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis by dynamic light scattering | Escherichia coli K-12 |
tetramer | 4 * 36000, detagged recombinant enzyme, SDS-PAGE | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the nucleoside hydrolase enzyme family | Escherichia coli K-12 |
pyrimidine nucleoside hydrolase | - |
Escherichia coli K-12 |
pyrimidine-specific nucleoside hydrolase | - |
Escherichia coli K-12 |
YeiK | - |
Escherichia coli K-12 |