Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.6 extracted from

  • Mather, I.H.; Knight, M.
    A heat-stable nicotinamide-adenine dinucleotide glycohydrolase from Pseudomonas putida KB1. Partial purification and some properties of the enzyme and an inhibitory protein (1972), Biochem. J., 129, 141-152.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.6

Inhibitors

Inhibitors Comment Organism Structure
additional information NADase inhibitor from Pseudomonas putida KB1 Pseudomonas putida KB1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 thio-NAD+
-
 Pseudomonas putida KB1
0.51
-
 nicotinamide-hypoxanthine dinucleotide phosphate
-
 Pseudomonas putida KB1
0.66
-
 NADP+
-
 Pseudomonas putida KB1
0.85
-
 NAD+
-
 Pseudomonas putida KB1
1.44
-
 nicotinamide-hypoxanthine dinucleotide
-
 Pseudomonas putida KB1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
 gel filtration Pseudomonas putida KB1

Organism

Organism UniProt Comment Textmining
Pseudomonas putida KB1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas putida KB1

Storage Stability

Storage Stability Organism
-20°C, stable for several weeks Pseudomonas putida KB1
4°C, 0.1 M Tris-HCl buffer, pH 7.0, 50% loss of activity Pseudomonas putida KB1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-NAD+ + H2O beta-NAD+ and NADP+ are hydrolyzed at equal rates Pseudomonas putida KB1 ?
-
 ?
NADP+ + H2O
-
 Pseudomonas putida KB1 nicotinamide + ADPribose-phosphate
-
 ?
nicotinamide-hypoxanthine dinucleotide + H2O
-
 Pseudomonas putida KB1 ?
-
 ?
nicotinamide-hypoxanthine dinucleotide phosphate + H2O
-
 Pseudomonas putida KB1 ?
-
 ?
thio-NAD+ + H2O
-
 Pseudomonas putida KB1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas putida KB1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 60% loss of activity after 30 min, 80% loss of activity after 1 h Pseudomonas putida KB1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 10 citrate buffer, activity is essentially unchanged Pseudomonas putida KB1
6 10 phosphate buffer, activity is essentially unchanged Pseudomonas putida KB1

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 10 5 min, 50 mM citrate, stable Pseudomonas putida KB1
4 5 5 min, less than 10% loss of activity Pseudomonas putida KB1