Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged wild-type and mutant enzymes in Pichia apstoris and secretion to the culture medium | Schistosoma mansoni |
Protein Variants | Comment | Organism |
---|---|---|
D133A | site-directed mutagenesis | Schistosoma mansoni |
E124A | site-directed mutagenesis, the mutant is not inhibited by cyanidin in contrast to the wild-type enzyme | Schistosoma mansoni |
E202A | site-directed mutagenesis, the mutant is much less inhibited by cyanidin compared to the wild-type enzyme | Schistosoma mansoni |
H103A | site-directed mutagenesis, the mutant is less inhibited by cyanidin compared to the wild-type enzyme | Schistosoma mansoni |
H103F | site-directed mutagenesis | Schistosoma mansoni |
S169A | site-directed mutagenesis | Schistosoma mansoni |
W165A | site-directed mutagenesis, the mutant is inhibited by cyanidin like the wild-type enzyme | Schistosoma mansoni |
W165F | site-directed mutagenesis | Schistosoma mansoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanidin | complete inhibition of the wild-type enzyme at 0.1 mM | Schistosoma mansoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Schistosoma mansoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + H2O | Schistosoma mansoni | - |
ADP-D-ribose + nicotinamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | Q32TF5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Pichia apstoris culture supernatant by nickel affinity chromatography | Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + H2O | - |
Schistosoma mansoni | ADP-D-ribose + nicotinamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD+ catabolizing enzyme | - |
Schistosoma mansoni |
SmNACE | - |
Schistosoma mansoni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Schistosoma mansoni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Schistosoma mansoni |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme shows significant structural and functional analogy to the members of the CD38/ADP-ribosyl cyclase family but a lack of ADP-ribosyl cyclase activity that might be ascribed to subtle changes in its active site. In sharp contrast with mammalian CD38, the signature Glu124 is as critical as Glu202 for catalysis by the parasite enzyme. Sequence comparisons | Schistosoma mansoni |
additional information | three dimensional homology modeling of the enzyme, very important role of Glu202 and of the hydrophobic domains overwhelmingly in the efficiency of the nicotinamide-ribosyl bond cleavage step. The nicotinamide-ribosyl bond is cleaved upon the first step of catalysis and is surrounded by three hydrophobic side chains Leu123, Tyr172 and Phe102 | Schistosoma mansoni |