Cloned (Comment) | Organism |
---|---|
the C-terminal glycohydrolase domain of SPN (residues 191-451) in complex with IFS is expressed in Escherichia coli strain DL41 | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
C-terminal glycohydrolase domain of SPN (residues 191-451) in complex with IFS, vapor diffusion method, using 20%-25% (w/v) PEG 8K and 100 mM Tris-HCl (pH 8.0), at 22°C | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity column chromatography, Q-Sepharose column chromatography, and S-200 gel filtration | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-NAD+ + H2O | - |
Streptococcus pyogenes | ADP-ribose + nicotinamide + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-NAD+ glycohydrolase | - |
Streptococcus pyogenes |
SPN | - |
Streptococcus pyogenes |