Cloned (Comment) | Organism |
---|---|
gene hvnA, part of a four gene operon, and monocistronic gene hvnB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha and BW23474 | Aliivibrio fischeri |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of inactive hvnA/hvnB null mutant | Aliivibrio fischeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | inhibits the reaction between ADP-ribose and polypeptides | Aliivibrio fischeri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion | Aliivibrio fischeri | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant enzyme HvnB, SDS-PAGE | Aliivibrio fischeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | Q56685 | isolated from Euprymna scolopes, genes hvnA and hvnB | - |
Aliivibrio fischeri | Q9F0R5 | isolated from Euprymna scolopes light organ, genes hvnA and hvnB | - |
Purification (Comment) | Organism |
---|---|
native enzymes partially from culture supernatant, recombinant enzymes from Escherichia coli | Aliivibrio fischeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,N6-etheno-NAD+ + H2O | - |
Aliivibrio fischeri | ? | - |
? | |
NAD+ + H2O | - |
Aliivibrio fischeri | ADP-ribose + nicotinamide | ADP-ribose reacts further with polyarginine, polyhistidine, or polylysine | ? | |
NAD+ + H2O | - |
Aliivibrio fischeri | ADP-ribose + nicotinamide | ADP-ribose reacts spontaneously with polyarginine, polyhistidine, or polylysine | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant enzyme HvnB, SDS-PAGE | Aliivibrio fischeri |
Synonyms | Comment | Organism |
---|---|---|
HvnA | - |
Aliivibrio fischeri |
HvnB | - |
Aliivibrio fischeri |
NAD+-glycohydrolase | - |
Aliivibrio fischeri |
NADase | - |
Aliivibrio fischeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aliivibrio fischeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aliivibrio fischeri |