Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.5 extracted from

  • Green, S.; Dobrjansky, A.
    Nicotinamide adenine dinucleotide glycohydrolases from Ehrlich ascites tumor cell nuclei: isolation, partial purification and properties (1972), Biochemistry, 11, 4108-4113.
    View publication on PubMed
Search for more literature for 3.2.2.5

General Stability

General Stability Organism
chromatin NADase is sensitiv to Dnase Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information nuclear membrane NADase is insensitiv to 1 M NaCl Mus musculus
NaCl 0.5 M or more, 65% loss in activity, irreversible, DNA associated NADase Mus musculus
nicotinamide 0.33 mM, 50% inhibition Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Mus musculus 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + H2O Mus musculus
-
 ADPribose + nicotinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + H2O
-
 Mus musculus ADPribose + nicotinamide
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 2 min, 100% inactivation, DNA associated NADase Mus musculus
45
-
 5 min, stable, nuclear membrane NADase Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 7 nuclear membrane Mus musculus
6.6 7.1 DNA associated NADase Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.33
-
 nicotinamide
-
 Mus musculus