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Literature summary for 3.2.2.30 extracted from

  • Mishra, V.; Ronning, D.R.
    Crystal structures of the Helicobacter pylori MTAN enzyme reveal specific interactions between S-adenosylhomocysteine and the 5-alkylthio binding subsite (2012), Biochemistry, 51, 9763-9772.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rosetta cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method. Inactive D198N mutant bound to 5'-methylthioadenosine using 0.2 M magnesium chloride, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350 Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
D198N inactive Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-amino-6-deoxyfutalosine + H2O Helicobacter pylori
-
 dehypoxanthine futalosine + adenine
-
 ?
additional information Helicobacter pylori 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosyl-L-homocysteine, 5'-methylthioadenosine, 5'-deoxyadenosine, and 6-amino-6-deoxyfutalosine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-amino-6-deoxyfutalosine + H2O
-
 Helicobacter pylori dehypoxanthine futalosine + adenine
-
 ?
additional information 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosyl-L-homocysteine, 5'-methylthioadenosine, 5'-deoxyadenosine, and 6-amino-6-deoxyfutalosine Helicobacter pylori ?
-
 ?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Helicobacter pylori
MTAN
-
 Helicobacter pylori