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Literature summary for 3.2.2.30 extracted from

  • Della Ragione, F.; Porcelli, M.; Carteni-Farina, M.; Zappia, V.
    Escherichia coli S-adenosylhomocysteine/5'-methylthioadenosine nucleosidasepurification, substrate specificity and mechanisms of action (1985), Biochem. J., 232, 335-341.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.30

Inhibitors

Inhibitors Comment Organism Structure
5'-Chloroformycin powerful inhibitor Escherichia coli
5'-isobutylthio-3-deaza-adenosine poor inhibitor Escherichia coli
5'-Isobutylthioinosine
-
 Escherichia coli
5'-methylthio-3-deaza-adenosine poor inhibitor Escherichia coli
5'-Methylthioformycin powerful inhibitor Escherichia coli
5'-methylthiotubercidin powerful inhibitor Escherichia coli
5'-n-butylthioinosine
-
 Escherichia coli
adenine poor inhibitor Escherichia coli
additional information not inhibited by 5'-methylthioinosine, S-inosyl-L-homocysteine, S-guanosyl-L-homocysteine, S-N6-methyl-3-deaza-adenosyl-L-homocysteine, S-adenosyl-L-homocysteine dialdehyde, acyclic adenosyl-L-homocysteine, S-cytidylhomocysteine, hypoxanthine, guanine, and AMP Escherichia coli
S-8-aza-adenosyl-L-homocysteine powerful inhibitor Escherichia coli
S-adenosyl-L-homocysteine sulfoxide poor inhibitor Escherichia coli
S-formycinylhomocysteine powerful inhibitor Escherichia coli
S-N6-dimethyl-3-deaza-adenosyl-L-homocysteine poor inhibitor Escherichia coli
S-Tubercidinylhomocysteine powerful inhibitor Escherichia coli
sinefungin poor inhibitor Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, hydroxyapatite column chromatography, S-formycinylhomocysteine-Sepharose column chromatography, and Sephacryl S-200 gel filtration Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer (pH 7.0), at least 2 months, no significant loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deaminated S-adenosyl-L-homocysteine + H2O 33% activity compared to 5'-methylthioadenosine Escherichia coli ?
-
 ?
decarboxylated S-adenosyl-L-homocysteine + H2O 18% activity compared to 5'-methylthioadenosine Escherichia coli ?
-
 ?
additional information no activity toward 5'-methylthioinosine, 5'-isobutylthioinosine, 5'-isobutylthio-3-deaza-adenosine, 5'-methylthio-3-deaza-adenosine, 5'-chloroformycin, 5'-methylthioformycin, 5'-ethylthiotubercidin, S-tubercidinylhomocysteine, S-inosyl-L-homocysteine, S-guanosyl-L-homocysteine, S-cytidylhomocysteine, S-8-aza adenosyl-L-homocysteine, S-n-methyl-3-deaza-adenosyl-L-homocysteine, S-n6-dimethyl-3-deaza-adenosyl-L-homocysteine, sinefungin, S-adenosyl-L-homocysteine dialdehyde, and acyclic adenosyl-L-homocysteine Escherichia coli ?
-
 ?
S-2-aza-adenosyl-L-homocysteine + H2O 27% activity compared to 5'-methylthioadenosine Escherichia coli ?
-
 ?
S-adenosyl-D-homocysteine + H2O 26% activity compared to 5'-methylthioadenosine Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
adoHcy/MTA nucleosidase
-
 Escherichia coli
S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme is rapidly inactivated after exposure for 15 min at 60°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000097
-
 S-formycinylhomocysteine in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli
0.000027
-
 5'-Methylthioformycin in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli
0.000322
-
 5'-Chloroformycin in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli
0.000928
-
 S-8-aza-adenosyl-L-homocysteine in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli
0.0019
-
 S-Tubercidinylhomocysteine in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli
0.0041
-
 5'-methylthiotubercidin in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00002
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli S-formycinylhomocysteine
0.00006
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-Methylthioformycin
0.0004
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-Chloroformycin
0.0018
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli S-8-aza-adenosyl-L-homocysteine
0.0032
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli S-Tubercidinylhomocysteine
0.0077
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-methylthiotubercidin
0.013
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-isobutylthio-3-deaza-adenosine
0.02
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli S-N6-dimethyl-3-deaza-adenosyl-L-homocysteine
0.024
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli sinefungin
0.069
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli S-adenosyl-L-homocysteine sulfoxide
0.079
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-methylthio-3-deaza-adenosine
0.094
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-n-butylthioinosine
0.132
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli 5'-Isobutylthioinosine
0.216
-
 in 50 mM potassium phosphate buffer, pH 7.0, at 37°C Escherichia coli adenine