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Literature summary for 3.2.2.3 extracted from

  • Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.
    Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis (2009), Plant Cell, 21, 876-891.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of URH1 in Arabidopsis thaliana plants under control of the CaMV 35S promoter Oryza sativa
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Arabidopsis thaliana
0.8
-
 uridine recombinant enzyme expressed in Escherichia coli Arabidopsis thaliana
2.1
-
 uridine recombinant enzyme expressed in Saccharomyces cerevisiae Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SJM7
-
-
Oryza sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
guard cell
-
 Arabidopsis thaliana
-
pollen
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
root tip
-
 Arabidopsis thaliana
-
vascular cell of roots and in root tips Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
303.3
-
 purified recombinant enzyme, substrate uridine Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine Arabidopsis thaliana ?
-
 ?
uridine + H2O
-
 Arabidopsis thaliana D-ribose + uracil
-
 ?

Synonyms

Synonyms Comment Organism
URH1
-
 Oryza sativa
URH1
-
 Arabidopsis thaliana
uridine-ribohydrolase 1
-
 Oryza sativa
uridine-ribohydrolase 1
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis thaliana, with a pivotal function of URH1 as regulative in pyrimidine degradation. The enzyme activity must be well balanced in the early phase of plant development Arabidopsis thaliana