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Literature summary for 3.2.2.3 extracted from

  • Belenky, P.; Christensen, K.C.; Gazzaniga, F.; Pletnev, A.A.; Brenner, C.
    Nicotinamide riboside and nicotinic acid riboside salvage in fungi and mammals. Quantitative basis for Urh1 and purine nucleoside phosphorylase function in NAD+ metabolism (2009), J. Biol. Chem., 284, 158-164.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
immucillin-H
-
 Saccharomyces cerevisiae
additional information Urh1 activity is unaffected by EDTA Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 nicotinamide riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
0.045
-
 nicotinic acid riboside methyl ester in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
0.15
-
 nicotinic acid riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
1.6
-
 uridine in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Urh1 activity is unaffected by the addition of MgCl2 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4742
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide riboside + H2O the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase Saccharomyces cerevisiae nicotinamide + D-ribose
-
 ?
nicotinamide riboside + H2O the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase Saccharomyces cerevisiae BY4742 nicotinamide + D-ribose
-
 ?
nicotinic acid riboside + H2O
-
 Saccharomyces cerevisiae nicotinic acid + D-ribose
-
 ?
nicotinic acid riboside + H2O
-
 Saccharomyces cerevisiae BY4742 nicotinic acid + D-ribose
-
 ?
nicotinic acid riboside methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
nicotinic acid riboside methyl ester + H2O
-
 Saccharomyces cerevisiae BY4742 ?
-
 ?
uridine + H2O
-
 Saccharomyces cerevisiae uracil + D-ribose
-
 ?
uridine + H2O
-
 Saccharomyces cerevisiae BY4742 uracil + D-ribose
-
 ?

Synonyms

Synonyms Comment Organism
URH1
-
 Saccharomyces cerevisiae
uridine-preferring pyrimidine nucleoside hydrolase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 nicotinic acid riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
2.3
-
 nicotinic acid riboside methyl ester in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
20
-
 uridine in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae
23.4
-
 nicotinamide riboside in 50 mM Tris-HCl, pH 7.0 Saccharomyces cerevisiae