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Literature summary for 3.2.2.29 extracted from

  • Morgan, M.T.; Bennett, M.T.; Drohat, A.C.
    Excision of 5-halogenated uracils by human thymine DNA glycosylase. Robust activity for DNA contexts other than CpG (2007), J. Biol. Chem., 282, 27578-27586.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.29

Cloned(Commentary)

Cloned (Comment) Organism
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 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-bromouracil-mismatched double-stranded DNA + H2O Homo sapiens potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions 5-bromouracil + double-stranded DNA with abasic site
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 ?
5-chlorouracil-mismatched double-stranded DNA + H2O Homo sapiens potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions 5-chlorouracil + double-stranded DNA with abasic site
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
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Purification (Commentary)

Purification (Comment) Organism
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 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromouracil-mismatched double-stranded DNA + H2O potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions Homo sapiens 5-bromouracil + double-stranded DNA with abasic site
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 ?
5-chlorouracil-mismatched double-stranded DNA + H2O potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions Homo sapiens 5-chlorouracil + double-stranded DNA with abasic site
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 ?
5-chlorouracil-mismatched double-stranded DNA + H2O removes a variety of damaged bases (X) with a preference for lesions in a CpG/X context. The maximal activity for G/X substrates depends significantly on the 5' base pair. The maximal activity decreases by 6fold, 11fold, and 82fold for TpG/5-chlorouracil, GpG/5-chlorouracil, and ApG/5-chlorouracil, respectively, as compared with CpG/5-chlorouracil. Human TDG activity is reduced 102.3–104.3fold for A/X relative to G/X pairs and reduced further for A/X pairs with a 5' pair other than C/G. The effect of altering the 5' pair and/or the opposing base (G/X versus A/X) is greater for substrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT). The largest CpG context effects are observed for the excision of thymine Homo sapiens 5-chlorouracil + double-stranded DNA with abasic site
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 ?
5-fluorouracil-mismatched double-stranded DNA + H2O the activity for G/5-fluorouracil, G/5-chlorouracil, and G/5-bromouracil, with any 5'-flanking pair, meets and in most cases significantly exceeds the CpG/T activity. Human TDG activity is reduced 102.3–104.3fold for A/X relative to G/X pairs and reduced further for A/X pairs with a 5' pair other than C/G. The effect of altering the 5' pair and/or the opposing base (G/X versus A/X) is greater for substrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT). The largest CpG context effects are observed for the excision of thymine Homo sapiens 5-fluorouracil + double-stranded DNA with abasic site
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 ?
thymine-mismatched double-stranded DNA + H2O thymine DNA glycosylase excises thymine from G/T mispairs. Human TDG activity is reduced 102.3–104.3fold for A/X relative to G/X pairs and reduced further for A/X pairs with a 5' pair other than C/G. The effect of altering the 5' pair and/or the opposing base (G/X versus A/X) is greater for substrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT). The largest CpG context effects are observed for the excision of thymine Homo sapiens thymine + double-stranded DNA with abasic site
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 ?

Synonyms

Synonyms Comment Organism
hTDG
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 Homo sapiens
thymine DNA glycosylase
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 Homo sapiens