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Literature summary for 3.2.2.29 extracted from
- The main role of human thymine-DNA glycosylase is removal of thymine produced by deamination of 5-methylcytosine and not removal of ethenocytosine (2003), J. Biol. Chem., 278, 8739-8744.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Homo sapiens | thymine-DNA glycosylase has a strong sequence preference for CpG sites in the excision of both thymine and ethenocytosine. This suggests a main role of thymine-DNA glycosylase in vivo is the removal of thymine produced by deamination of 5-methylcytosine at CpG sites | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q13569 | recombinant | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | ethenocytosine base-paired with guanine within a CpG site (i.e. CpG-ethenocytosine-DNA) is by far the best substrate. The next best substrates are DNA duplexes containing TpG/ethenocytosine, GpG/ethenocytosine, and CpG/T. The worst substrates are DNA duplexes containing ApG/ethenocytosine and TpG/T. DNA containing ethenocytosine is bound much more tightly than DNA containing a G/T mismatch | Homo sapiens | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
| double-stranded DNA + H2O | thymine-DNA glycosylase has a strong sequence preference for CpG sites in the excision of both thymine and ethenocytosine. This suggests a main role of thymine-DNA glycosylase in vivo is the removal of thymine produced by deamination of 5-methylcytosine at CpG sites | Homo sapiens | ? | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | ethenocytosine base-paired with guanine within a CpG site (i.e. CpG-ethenocytosine-DNA) is by far the best substrate. The next best substrates are DNA duplexes containing TpG/ethenocytosine, GpG/ethenocytosine, and CpG/T. The worst substrates are DNA duplexes containing ApG/ethenocytosine and TpG/T. DNA containing ethenocytosine is bound much more tightly than DNA containing a G/T mismatch | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thymine-DNA glycosylase | - |
Homo sapiens |
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