Literature summary for 3.2.2.29 extracted from

Neddermann, P.; Gallinari, P.; Lettieri, T.; Schmid, D.; Truong, O.; Hsuan, J.J.; Wiebauer, K.; Jiricny, J.
Cloning and expression of human G/T mismatch-specific thymine-DNA glycosylase (1996), Biol. Chem., 271, 12767-12774.

Search for more literature for 3.2.2.29

Cloned(Commentary)

Cloned (Comment)	Organism
expression in in cell-free extract and in Escherichia coli	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 60000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13569	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thymine-mismatched double-stranded DNA + H2O	the recombinant enzyme shows a significant preference for G/T mispairs in a CpG context. The enzyme is also capable of processing mismatches between thymine and 6-O-methylguanine, whereby it generates an apyrimidinic site opposite the modified guanine. TDG preferentially addresses 6-O-methyl G/T mispairs when the neighboring base 5' to the 6-O-methyl G is a C rather than a G. TDG is the only enzyme present in the HeLa nuclear extracts capable of processing G/T mispairs in oligonucleotide substrate	Homo sapiens	thymine + double-stranded DNA with abasic site	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
G/T glycosylase	-	Homo sapiens
mismatch-specific thymine-DNA glycosylase	-	Homo sapiens
TDG	-	Homo sapiens
thymine-DNA glycosylase	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)