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Literature summary for 3.2.2.27 extracted from

  • Assefa, N.G.; Niiranen, L.; Johnson, K.A.; Leiros, H.K.; Smalas, A.O.; Willassen, N.P.; Moe, E.
    Structural and biophysical analysis of interactions between cod and human uracil-DNA N-glycosylase (UNG) and UNG inhibitor (Ugi) (2014), Acta Crystallogr. Sect. D, 70, 2093-2100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Homo sapiens
expressed in Escherichia coli BL21(DE3) pLysS cells Gadus morhua

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a bacteriophage encoded natural inhibitor, hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.4, 270 mM Li2SO4, 4% (w/v) PEG 550 MME, 17% (w/v) PEG 4000 Gadus morhua
in complex with a bacteriophage encoded natural inhibitor, hanging drop vapor diffusion method, using 0.1 M Tris–HCl pH 7.4, 270 mM Li2SO4, 4% (w/v) PEG 550 MME, 17% (w/v) PEG 4000 Homo sapiens

Organism

Organism UniProt Comment Textmining
Gadus morhua Q9I983
-
-
Homo sapiens P13051
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and Superdex 75 gel filtration Homo sapiens
Q-Sepharose column chromatography and Superdex 75 gel filtration Gadus morhua

Synonyms

Synonyms Comment Organism
UNG
-
Homo sapiens
UNG
-
Gadus morhua
uracil-DNA N-glycosylase
-
Homo sapiens
uracil-DNA N-glycosylase
-
Gadus morhua