Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21 (DE3) ung-151 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D173F | site-directed mutagenesis | Arabidopsis thaliana |
D173R | site-directed mutagenesis | Arabidopsis thaliana |
additional information | the Arabidopsis mutant line GK-440E07 harbors a T-DNA insertion in the AtUNG gene. AtUNG-deficient plants do not display any apparent phenotype, but show increased resistance to 5-fluorouracil. The resistance of atung-/- mutants to 5-FU is accompanied by the accumulation of uracil residues in DNA | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | AtUNG is the major UDG activity in Arabidopsis thaliana AtUNG excises uracil in vivo but generates toxic AP sites when processing abundant U:A pairs in dTTP-depleted cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21 (DE3) ung-151 by nickel affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | AtUNG is the major UDG activity in Arabidopsis thaliana AtUNG excises uracil in vivo but generates toxic AP sites when processing abundant U:A pairs in dTTP-depleted cells | Arabidopsis thaliana | ? | - |
? | |
additional information | AtUNG exhibits the narrow substrate specificity and single-stranded DNA preference, it is active on 5'-labeled 51mer oligonucleotide duplex containing a U:G mispair. AtUNG is significantly more active on U:G mispairs than on U:A pairs. The activity on U:T and U:C mispairs is slightly lower than on U:G, but higher than on U:A pairs | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | AtUng is a plant member of the family-1 of UDGs typified by Escherichia coli Ung | Arabidopsis thaliana |
UNG | - |
Arabidopsis thaliana |
uracil DNA glycosylase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | assay at | Arabidopsis thaliana |