Application | Comment | Organism |
---|---|---|
drug development | inhibitor design and development, inhibition of UNG could be a rational strategy for the treatment of infections with poxviruses | Vaccinia virus |
Cloned (Comment) | Organism |
---|---|
expression of the His-tagged viral enzyme in the UNG-deficient Escherichia coli K12 strain MS1021 | Vaccinia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | strong inhibition at 7.5 mM | Vaccinia virus | |
additional information | no inhibition by uracil-DNA glycosylase inhibitor, UGI, from Bacillus subtilis bacteriophages PBS1 and PBS2 | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Vaccinia virus | the DNA repair protein uracil-DNA glycosylase is one of the viral enzymes important for poxvirus pathogenesis, it is part of the base excision repair pathway, BER | uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged viral enzyme from UNG-deficient Escherichia coli K12 strain MS1021 by nickel affinity, hydropohobic interaction, and anion exchange chromatography | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | the DNA repair protein uracil-DNA glycosylase is one of the viral enzymes important for poxvirus pathogenesis, it is part of the base excision repair pathway, BER | Vaccinia virus | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | UNG excises uracil from DNA, preferentially when it is opposite to cytosine | Vaccinia virus | uracil + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UNG | - |
Vaccinia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vaccinia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Vaccinia virus |