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Literature summary for 3.2.2.27 extracted from

  • Scaramozzino, N.; Sanz, G.; Crance, J.M.; Saparbae, M.; Drillien, R.; Laval, J.; Kavli, B.; Garin, D.
    Characterisation of the substrate specificity of homogeneous vaccinia virus uracil-DNA glycosylase (2003), Nucleic Acids Res., 31, 4950-4957.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.27

Application

Application Comment Organism
drug development inhibitor design and development, inhibition of UNG could be a rational strategy for the treatment of infections with poxviruses Vaccinia virus

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged viral enzyme in the UNG-deficient Escherichia coli K12 strain MS1021 Vaccinia virus

Inhibitors

Inhibitors Comment Organism Structure
MgCl2 strong inhibition at 7.5 mM Vaccinia virus
additional information no inhibition by uracil-DNA glycosylase inhibitor, UGI, from Bacillus subtilis bacteriophages PBS1 and PBS2 Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Vaccinia virus the DNA repair protein uracil-DNA glycosylase is one of the viral enzymes important for poxvirus pathogenesis, it is part of the base excision repair pathway, BER uracil + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged viral enzyme from UNG-deficient Escherichia coli K12 strain MS1021 by nickel affinity, hydropohobic interaction, and anion exchange chromatography Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O the DNA repair protein uracil-DNA glycosylase is one of the viral enzymes important for poxvirus pathogenesis, it is part of the base excision repair pathway, BER Vaccinia virus uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O UNG excises uracil from DNA, preferentially when it is opposite to cytosine Vaccinia virus uracil + double-stranded DNA with abasic site
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 ?

Synonyms

Synonyms Comment Organism
UNG
-
 Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Vaccinia virus