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Literature summary for 3.2.2.27 extracted from

  • Darwanto, A.; Theruvathu, J.A.; Sowers, J.L.; Rogstad, D.K.; Pascal, T.; Goddard III, W.; Sowers, L.C.
    Mechanisms of base selection by human single-stranded selective monofunctional uracil-DNA glycosylase (2009), J. Biol. Chem., 284, 15835-15846.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
SMUG1 is encoded within a small region of human chromosome 12, cloning from HeLa S3 cells, expression of GST-tagged SMUG1 in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of SMUG1 by uracil glycosylase inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information SMUG1 single-turnover kinetics using oligonucleotide substrates containing purine and pyrimidine analogs Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview ?
-
?
uracil-mismatched double-stranded DNA + H2O Homo sapiens
-
uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O Homo sapiens
-
uracil + single-stranded DNA with abasic site
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q53HV7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged SMUG1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa-S3 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview Homo sapiens ?
-
?
additional information preference of hSMUG1 for mispaired uracil over uracil paired with adenine, substrate selectivity with oligonucleotide 24-mers containing uracil with different 5-substituents, overview Homo sapiens ?
-
?
uracil-mismatched double-stranded DNA + H2O
-
Homo sapiens uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O
-
Homo sapiens uracil + single-stranded DNA with abasic site
-
?

Synonyms

Synonyms Comment Organism
single-strand selective monofunctional uracil-DNA glycosylase
-
Homo sapiens
SMUG1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens