Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-Methylhypoxanthine | 50% inhibition at 5 mM, no inhibition at 1 mM | Camellia sinensis | |
7-methylxanthine | 40% inhibition at 5 mM, no inhibition at 1 mM | Camellia sinensis | |
Ca2+ | slight inhibition | Camellia sinensis | |
Cu2+ | slight inhibition | Camellia sinensis | |
EDTA | 90 inhibition at 1 mM | Camellia sinensis | |
additional information | no inhibitin by Mg2+, Fe2+, Co2+, NaF, and iodoacetate | Camellia sinensis | |
PCMB | 80% inhibition at 0.5 mM | Camellia sinensis | |
Zn2+ | slight inhibition | Camellia sinensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Camellia sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylxanthosine + H2O | Camellia sinensis | second step in caffeine biosynthesis, pathway overview | 7-methylxanthine + D-ribose | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from leaves, separated from adenosine nucleosidase, EC 3.2.2.7, by anion exchange chromatography, followed by hydroxylapatite chromatography and gel filtration, to homogeneity | Camellia sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00656 | - |
partially purified enzyme, after anion exchange chromatography | Camellia sinensis |
0.0294 | - |
purified enzyme | Camellia sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyladenosine + H2O | 22% of the activity with 7-methylxanthosine, substrate as 7-methyladenosine perchlorate | Camellia sinensis | 1-methyladenine + D-ribose | - |
ir | |
1-methylinosine + H2O | 9% of the activity with 7-methylxanthosine | Camellia sinensis | 1-methylhypoxanthine + D-ribose | - |
ir | |
1-methylxanthosine + H2O | 12% of the activity with 7-methylxanthosine | Camellia sinensis | 1-methylxanthine + D-ribose | - |
ir | |
3-methyladenosine + H2O | 178% of the activity with 7-methylxanthosine, substrate as 3-methyladenosine p-toluenesulfonate | Camellia sinensis | 3-methyladenine + D-ribose | - |
ir | |
3-methylguanosine + H2O | 38% of the activity with 7-methylxanthosine | Camellia sinensis | 3-methylguanine + D-ribose | - |
ir | |
3-methylinosine + H2O | 128% of the activity with 7-methylxanthosine | Camellia sinensis | 3-methylhypoxanthine + D-ribose | - |
ir | |
3-methylxanthosine + H2O | 198% of the activity with 7-methylxanthosine | Camellia sinensis | 3-methylxanthine + D-ribose | - |
ir | |
7-methyladenosine + H2O | 13% of the activity with 7-methylxanthosine | Camellia sinensis | 7-methyladenine + D-ribose | - |
ir | |
7-methylguanosine + H2O | 189% of the activity with 7-methylxanthosine | Camellia sinensis | 7-methylguanine + D-ribose | - |
ir | |
7-methylinosine + H2O | 121% of the activity with 7-methylxanthosine | Camellia sinensis | 7-methylhypoxanthine + D-ribose | - |
ir | |
7-methylxanthosine + H2O | second step in caffeine biosynthesis, pathway overview | Camellia sinensis | 7-methylxanthine + D-ribose | - |
ir | |
7-methylxanthosine + H2O | the reverse reaction is catalyzed by a nucleoside phosphorylase using ribose-1-phosphate, overview | Camellia sinensis | 7-methylxanthine + D-ribose | - |
ir | |
additional information | substrate specificity, overview, no activity with 1-methylguanosine and guanosine, poor activity with inosine and adenosine | Camellia sinensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-MeNase | - |
Camellia sinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Camellia sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Camellia sinensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the purified enzyme is unstable in acidic medium | Camellia sinensis |