Cloned (Comment) | Organism |
---|---|
P2E, P2T and P2G mutant Fpg are cloned and expressed in Escherichia coli BH20(fpg-) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
P2E | completely inactive mutant: no 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine DNA/7,8-dihydro-8-oxoguanine-DNA glycosylase activity or cleavage of DNA containing AP sites | Escherichia coli |
P2G | mutant with 10% of wild-type 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine DNA glycosylase activity and barely detectable 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no cleavage of DNA containing AP sites | Escherichia coli |
P2T | mutant with 10% of wild-type 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine DNA glycosylase activity and barely detectable 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no cleavage of DNA containing AP sites | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | possesses a zinc finger | Escherichia coli | |
Zn2+ | contains one zinc per enzyme molecule | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30200 | - |
1 * 30200, globular monomer | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | removes oxidized purines from oxidatively damaged DNA | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | Escherichia coli | dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair | 8-hydroxyguanine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type, P2G, P2E and P2T mutant Fpg | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine | catalytic mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions | Escherichia coli | ? | - |
? | |
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli | ? | - |
? | |
DNA + H2O | catalytic mechanism involves formation of Schiff base intermediate between DNA containing an oxidized residue and the N-terminal Pro-2 of Fpg, mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase and AP lyase activity, but less in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase activity | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 7,8-dihydro-8-oxoguanine-DNA | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | less mendatory role of P-2 in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity | Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? | |
additional information | not: dublex DNA containing a single tetrahydrofuran residue | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30200, globular monomer | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | - |
- |
8.6 |