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Literature summary for 3.2.2.23 extracted from

  • Boiteux, S.; O'Connor, T.R.; Laval, J.
    Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein (1987), EMBO J., 6, 3177-3183.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
fpg structural gene is cloned and sequenced, overexpression in Escherichia coli HB101 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30200
-
x * 30200, calculated from the amino acid sequence Escherichia coli
31000
-
x * 31000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli HB1100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
63.6fold, from overexpressing strain HB101 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
0.0146
-
pH 7.6, 37°C, enzyme from overexpressing strain HB101 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing ring-opened N7-methylguanine residues + H2O enzyme excises the secondary alkylation product of 7-methylguanine Fapy Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O enzyme excises the secondary alkylation product of 7-methylguanine Fapy Escherichia coli HB1100 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Escherichia coli
? x * 30200, calculated from the amino acid sequence Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Escherichia coli