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Literature summary for 3.2.2.22 extracted from
- Molecular cloning and functional analysis of a recombinant ribosome-inactivating protein (alpha-momorcharin) from Momordica charantia (2012), Appl. Microbiol. Biotechnol., 96, 939-950.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3) pLysS cells | Momordica charantia |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Momordica charantia |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Momordica charantia |
| 29094 | - |
x * 29094, calculated from amino acid sequence | Momordica charantia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28S rRNA + H2O | Momordica charantia | - |
Endo's fragment + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Momordica charantia | P16094 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Momordica charantia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28S rRNA + H2O | - |
Momordica charantia | Endo's fragment + ? | - |
? | |
| additional information | alpha-momorcharin has both N-glycosidase activity and DNA-nuclease activity | Momordica charantia | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Momordica charantia |
| ? | x * 29094, calculated from amino acid sequence | Momordica charantia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-MC | - |
Momordica charantia |
| alpha-momorcharin | - |
Momordica charantia |
| ribosome-inactivating protein | - |
Momordica charantia |
| RIP | - |
Momordica charantia |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | alpha-momorcharin can inhibit the mycelial growth of Fusarium solani, Fusarium oxysporum, and Pseudomonas aeruginosa | Momordica charantia |
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