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Literature summary for 3.2.2.22 extracted from
- Comparative analysis of depurination catalyzed by ricin A-chain on synthetic 32mer and 25mer oligoribonucleotides mimicking the sarcin/ricin domain of the rat 28S rRNA and E. coli 23S rRNA (2009), J. Biotechnol., 139, 156-162.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Ricinus communis | |
| 0.00457 | - |
sarcin/ricin domains of Rattus norvegicus 28S rRNA | pH 5.0 | Ricinus communis | |
| 0.00464 | - |
sarcin/ricin domains of Escherichia coli 23S rRNA | pH 5.0 | Ricinus communis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ricinus communis | ricin A-chain can inactivate eukaryotic ribosomes, but exhibits no N-glycosidase activity on intact Escherichia coli ribosomes | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Ricinus communis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | ricin A-chain can inactivate eukaryotic ribosomes, but exhibits no N-glycosidase activity on intact Escherichia coli ribosomes | Ricinus communis | ? | - |
? | |
| additional information | ricin-A-chain catalyzes the depurination of synthetic 32mer and 25mer oligoribonucleotides mimicking the sarcin/ricin domain of the Rattus norvegicus 28S rRNA and Escherichia coli 23S rRNA, comparative analysis, overview. Synthesis of synthetic 5'-FAM-fluorescence-labeled GAGA tetraloop oligoribonucleotide substrates, substrate recognition and binding, dynamics, overview | Ricinus communis | ? | - |
? | |
| sarcin/ricin domains of Escherichia coli 23S rRNA + H2O | - |
Ricinus communis | ? | - |
? | |
| sarcin/ricin domains of Rattus norvegicus 28S rRNA + H2O | - |
Ricinus communis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-glycosidase | - |
Ricinus communis |
| ricin-A-chain | - |
Ricinus communis |
| RTA | - |
Ricinus communis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | assay at | Ricinus communis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
sarcin/ricin domains of Escherichia coli 23S rRNA | pH 5.0 | Ricinus communis | |
| 0.0018 | - |
sarcin/ricin domains of Rattus norvegicus 28S rRNA | pH 5.0 | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Ricinus communis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | pH-dependence of the reaction, overview | Ricinus communis |
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