Literature summary for 3.2.2.22 extracted from

Shahidi-Noghabi, S.; Van Damme, E.J.; Smagghe, G.
Carbohydrate-binding activity of the type-2 ribosome-inactivating protein SNA-I from elderberry (Sambucus nigra) is a determining factor for its insecticidal activity (2008), Phytochemistry, 69, 2972-2978.

Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment)	Organism
cauliflower mosaic virus promoter	Sambucus nigra

Protein Variants

Protein Variants	Comment	Organism
D231E	B chain mutation in one carbohydrate binding site, reduced insecticidal activity, recombinant tobacco plant mutants SNA-I 103M1 and SNA-I 107M1	Sambucus nigra
N48S/D231E	B chain mutation in both carbohydrate binding sites almost abolished insecticidal activity comparable to control plants, recombinant tobacco plant mutants SNA-I 109M2 and SNA-I 112M2	Sambucus nigra

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Sambucus nigra	doses of more than 100 microg/ml SNA-I fed to pea aphids are toxic: LC50 = 374 microg/ml, doses of 100 and 50 microg/ml SNA-I reduce fecundity of pea aphids by about 70% and 43%, respectively, reduced survival and fecundity of adult tobacco aphids feeding on transgenic single SNA-I mutant tobacco plants (Nicotiana tabacum cv Samsun NN), not on double mutants that have no effect comparable to control plants that do not express SNA-I	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Sambucus nigra	-	elderberry, transgenic tobacco plants' action on hemipteran insects pea aphid Acyrthosiphon pisum and tobacco aphid Myzus nicotianae	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography on fetuin-Sepharose 4B and gel filtration of homogenized elderberry bark, homogenized leaves of tobacco plants centrifuged	Sambucus nigra

Source Tissue

Source Tissue	Comment	Organism	Textmining
bark	-	Sambucus nigra	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	doses of more than 100 microg/ml SNA-I fed to pea aphids are toxic: LC50 = 374 microg/ml, doses of 100 and 50 microg/ml SNA-I reduce fecundity of pea aphids by about 70% and 43%, respectively, reduced survival and fecundity of adult tobacco aphids feeding on transgenic single SNA-I mutant tobacco plants (Nicotiana tabacum cv Samsun NN), not on double mutants that have no effect comparable to control plants that do not express SNA-I	Sambucus nigra	?	-	?

Synonyms

Synonyms	Comment	Organism
Sambucus nigra agglutinin I	-	Sambucus nigra
SNA-I	-	Sambucus nigra
type-2 ribosome-inactivating protein	-	Sambucus nigra

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Release 2023.1 (January 2023)