Literature summary for 3.2.2.22 extracted from

Mayerhofer, P.U.; Cook, J.P.; Wahlman, J.; Pinheiro, T.T.; Moore, K.A.; Lord, J.M.; Johnson, A.E.; Roberts, L.M.
Ricin A chain insertion into endoplasmic reticulum membranes is triggered by a temperature increase to 37 {degrees}C (2009), J. Biol. Chem., 284, 10232-10242.

Search for more literature for 3.2.2.22

Protein Variants

Protein Variants	Comment	Organism
C171A	does not infer with enzymatic depurination activity, leaving C259 as docking station for the fluorescence dye, which changes its emission properties upon change from aqueous to hydrophobic (membrane) environment, C259 is reduced in the lumen of the endoplasmic reticulum	Ricinus communis
E177D/C259S/I249C	residues 249 and 259 show membrane- and temperature-induced structural transition, meaning that the residues are exposed to the bilayer interior, both labeled with N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine	Ricinus communis
K4R/C171A/E177D/K239R/E135K	no temperature-induced change, meaning that residue 135 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate	Ricinus communis
K4R/C171A/E177D/K239R/E61K	no temperature-induced change, meaning that residue 61 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate	Ricinus communis
K4R/C171A/E177D/K239R/Q128K	no temperature-induced change, meaning that residue 128 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate	Ricinus communis
K4R/C171A/E177D/K239R/Q98K	no temperature-induced change, meaning that residue 98 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate	Ricinus communis
K4R/C171A/E177D/K239R/R114K	no temperature-induced change, meaning that residue 114 is bound within the membrane right away, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate	Ricinus communis
K4R/C171A/E177D/K239R/R31K	residues 31 shows membrane- and temperature-induced structural transition, meaning that the residues are exposed to the bilayer interior, labeled with succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate	Ricinus communis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	stably membrane-bound at 37°C, even under sodium carbonate treatment at pH 11.5, extractable at 20°C	Ricinus communis	5783	-

Organism

Organism	UniProt	Comment	Textmining
Ricinus communis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	temperature induced major structural rearrangements of endoplasmic reticulum membrane-bound toxin at 37°C: loss of helical content, increase of beta sheets, C-terminus moves closer to membrane surface, insertion into the bilayer	Ricinus communis

Purification (Commentary)

Purification (Comment)	Organism
according to Deeks et al., Biochemistry 41, p. 3405-3413, 2002	Ricinus communis

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
rRNA + H2O	-	Ricinus communis	?	-	?

Synonyms

Synonyms	Comment	Organism
ricin toxin A-chain	-	Ricinus communis
RTA	-	Ricinus communis

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Release 2023.1 (January 2023)