Crystallization (Comment) | Organism |
---|---|
dynamics calculations suggest that steric factors cause the nucleoside to bind in an orientation where the enzyme destabilizes the formation of the oxacarbeniumion and thus precludes catalysis | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA | i.e. 8VdA-10, active site analogue, no substrate. Role of residue R180 in oxacarbenium ion destabilization, adenine is released from a second site within the molecule | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phytolacca americana | - |
isoform pokeweed antiviral protein, i.e. PAP | - |
Ricinus communis | P02879 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA + H2O | - |
Phytolacca americana | 8-vinyladenine + ? | slow | ? | |
additional information | enzyme catalyzes the release of two adenine residues | Ricinus communis | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA | 37°C, pH 4.0 | Ricinus communis |