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Literature summary for 3.2.2.22 extracted from

  • Zhang, C.; Medina-Bolivar, F.; Buswell, S.; Cramer, C.L.
    Purification and stabilization of ricin B from tobacco hairy root culture medium by aqueous two-phase extraction (2005), J. Biotechnol., 117, 39-48.
    View publication on PubMed
Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment) Organism
expression of ricin B-subunit in Nicotiana tabacum hairy roots, the recombinant protein is secreted to the culture medium Ricinus communis

General Stability

General Stability Organism
the recombinant secreted ricin-B expressed in tobacco hairy root culture is degraded in the medium within 24 h with complete loss after 72 h, the recombinant ricin-B can be recovered by aqueous two-phase extraction using PEG/potassium phosphate or PEG/sodium sulfate, the latter is more efficient Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted ricin-B from tobacco hairy root culture medium Ricinus communis

Renatured (Commentary)

Renatured (Comment) Organism
the recombinant secreted ricin-B expressed in tobacco hairy root culture is degraded in the medium within 24 h with complete loss after 72 h, the recombinant ricin-B can be recovered by aqueous two-phase extraction using PEG/potassium phosphate or PEG/sodium sulfate, the latter is more efficient Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining

Subunits

Subunits Comment Organism
dimer heterodimer formed by a catalytic subunit A, RTA, and a carbohydrate binding subunit B, RTB Ricinus communis

Synonyms

Synonyms Comment Organism
ricin
-
 Ricinus communis
type II ribosome-inactivating protein
-
 Ricinus communis