Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mirabilis expansa | - |
type I ribosome-inactivating protein ME1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from storage roots by immunoaffinity chromatography using a polyclonal anti-ME1 antibody resin | Mirabilis expansa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | storage root | Mirabilis expansa | - |
storage tissue | - |
Mirabilis expansa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
28S rRNA + H2O | substrate is from Saccharomyces cerevisiae YPH500 ribosomes, depurination of an adenine of the 28S rRNA, substrate structure oveview | Mirabilis expansa | ? | - |
? | |
additional information | substrate specificity, no activity with double-stranded heat-denatured DNA, the N-glycosidase activity of the ribosome-inactivating protein ME1 targets single-stranded regions of nucleic acids independent of sequence or structural motifs | Mirabilis expansa | ? | - |
? | |
non-ribosomal RNA + H2O | e.g. mRNA: Phytophthora cryptotogea B26 RNA, or Arabidopsis thaliana RNA, single-stranded, identification of cleavage sites, overview | Mirabilis expansa | ? | - |
? | |
single-stranded DNA + H2O | at 90°C heat-denatured substrate | Mirabilis expansa | ? | - |
? | |
un-capped RNA + H2O | - |
Mirabilis expansa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ME1 | - |
Mirabilis expansa |
RNA N-glycosidase | - |
Mirabilis expansa |
type I ribosome-inactivating protein | - |
Mirabilis expansa |
type I RIP | - |
Mirabilis expansa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mirabilis expansa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Mirabilis expansa |