Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.22 extracted from

  • Park, S.W.; Vepachedu, R.; Owens, R.A.; Vivanco, J.M.
    The N-glycosidase activity of the ribosome-inactivating protein ME1 targets single-stranded regions of nucleic acids independent of sequence or structural motifs (2004), J. Biol. Chem., 279, 34165-34174.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Mirabilis expansa
-
type I ribosome-inactivating protein ME1
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from storage roots by immunoaffinity chromatography using a polyclonal anti-ME1 antibody resin Mirabilis expansa

Source Tissue

Source Tissue Comment Organism Textmining
root storage root Mirabilis expansa
-
storage tissue
-
Mirabilis expansa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
28S rRNA + H2O substrate is from Saccharomyces cerevisiae YPH500 ribosomes, depurination of an adenine of the 28S rRNA, substrate structure oveview Mirabilis expansa ?
-
?
additional information substrate specificity, no activity with double-stranded heat-denatured DNA, the N-glycosidase activity of the ribosome-inactivating protein ME1 targets single-stranded regions of nucleic acids independent of sequence or structural motifs Mirabilis expansa ?
-
?
non-ribosomal RNA + H2O e.g. mRNA: Phytophthora cryptotogea B26 RNA, or Arabidopsis thaliana RNA, single-stranded, identification of cleavage sites, overview Mirabilis expansa ?
-
?
single-stranded DNA + H2O at 90°C heat-denatured substrate Mirabilis expansa ?
-
?
un-capped RNA + H2O
-
Mirabilis expansa ?
-
?

Synonyms

Synonyms Comment Organism
ME1
-
Mirabilis expansa
RNA N-glycosidase
-
Mirabilis expansa
type I ribosome-inactivating protein
-
Mirabilis expansa
type I RIP
-
Mirabilis expansa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mirabilis expansa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Mirabilis expansa