Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.22 extracted from

  • Habuka, N.; Miyano, M.; Kataoka, J.; Tsuge, H.; Noma, M.
    Specificities of RNA N-glycosidase activity of Mirabilis antiviral protein variants (1992), J. Biol. Chem., 267, 7758-7760.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
synthetic gene variants expressed in Escherichia coli MM294 Mirabilis jalapa

Protein Variants

Protein Variants Comment Organism
E168D replacing DNA fragments of the total synthetic gene of MAP, weaker inhibitory effect Mirabilis jalapa
R26L replacing DNA fragments of the total synthetic gene of MAP Mirabilis jalapa
R48L replacing DNA fragments of the total synthetic gene of MAP Mirabilis jalapa
Y118F replacing DNA fragments of the total synthetic gene of MAP, slightly stronger inhibitory effect Mirabilis jalapa
Y72F replacing DNA fragments of the total synthetic gene of MAP, slightly stronger inhibitory effect Mirabilis jalapa

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Mirabilis jalapa inhibits mechanical transmission of plant viruses such as tobacco mosaic virus ?
-
?

Organism

Organism UniProt Comment Textmining
Mirabilis jalapa
-
Mirabilis antiviral protein, MAP, MAP variants can maintain their N-glycosidase activity when the conserved Glu-168 and Arg-171 are changed to similar charged residues
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant proteins Mirabilis jalapa

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Mirabilis jalapa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information inactivates both eukaryotic and procaryotic ribosomes by means of site-specific RNA N-glycosidase activity, inhibits mechanical transmission of plant viruses Mirabilis jalapa ?
-
?
rRNA + H2O
-
Mirabilis jalapa ?
-
?
rRNA + H2O inhibits mechanical transmission of plant viruses such as tobacco mosaic virus Mirabilis jalapa ?
-
?

Synonyms

Synonyms Comment Organism
MAP MAP, a MAP variant with Arg-26 changed to Leu is designated R26L, a MAP variant with Arg-28 changed to Leu is designated R28L Mirabilis jalapa