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Literature summary for 3.2.2.21 extracted from
- Mutation of a unique aspartate residue abolishes the catalytic activity but not substrate binding of the mouse N-methylpurine-DNA glycosylase (MPG) (2000), J. Biol. Chem., 275, 4278-4282.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned and expressed in Escherichia coli BL21(DE3) | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D112N | site-directed mutagenesis | Mus musculus |
| D152N | site-directed mutagenesis | Mus musculus |
| D252N | site-directed mutagenesis | Mus musculus |
| D259N | site-directed mutagenesis | Mus musculus |
| D260N | site-directed mutagenesis | Mus musculus |
| D310N | site-directed mutagenesis | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | Mus musculus | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? |  |
| alkylated DNA + H2O | Homo sapiens | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Mus musculus | - |
mouse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, expressed in Escherichia coli | Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000008 | - |
mutant Asn-152, expressed in Escherichia coli | Mus musculus |
| 0.000005 | - |
mutant Asn-252, expressed in Escherichia coli | Mus musculus |
| 0.000007 | - |
mutant Asn-255 and Asn-231, expressed in Escherichia coli | Mus musculus |
| 0.000009 | - |
mutant Asn-259, expressed in Escherichia coli | Mus musculus |
| 0.00001 | - |
mutant Asn-260, expressed in Escherichia coli | Mus musculus |
| 0.000013 | - |
mutant Asn-112, expressed in Escherichia coli | Mus musculus |
| 0.000014 | - |
wild-type Ndelta100Cdelta18, expressed in Escherichia coli | Mus musculus |
| 0.000018 | - |
mutant Asn-310, expressed in Escherichia coli | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | - |
Mus musculus | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
| alkylated DNA + H2O | - |
Homo sapiens | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPG | - |
Mus musculus |
| MPG | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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