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Literature summary for 3.2.2.21 extracted from

  • Riazuddin, S.; Athar, A.; Ahmed, Z.; Lali, S.M.; Sohail, A.
    DNA glycosylase enzymes induced during chemical adaptation of M. luteus (1987), Nucleic Acids Res., 15, 6607-6624.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
spermidine stimulates activity Escherichia coli
spermine stimulates activity Escherichia coli

General Stability

General Stability Organism
very unstable Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2
-
Micrococcus luteus
additional information resistant to product inhibition by free 3-methyladenine Escherichia coli
N-ethylmaleimide
-
Escherichia coli
N-ethylmaleimide
-
Micrococcus luteus
NaCl
-
Escherichia coli
p-mercuribenzoate
-
Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no obligatory requirement, but addition of 1.5 mM MgCl2 causes stimulation in the catalytic activity 2fold Micrococcus luteus
Mg2+ stimulated by 1.5 mM MgCl2 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
gel filtration, Svedberg equation Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O Micrococcus luteus cellular repair of alkylated DNA base modifications 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000375
-
-
Micrococcus luteus

Storage Stability

Storage Stability Organism
-20°C, stored in 50% glycerol, undergoes complete inactivation in 3-4 weeks Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O
-
Micrococcus luteus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O cellular repair of alkylated DNA base modifications Micrococcus luteus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O
-
Micrococcus luteus 3-methyladenine + 3-methylguanine + 7-methyladenine + 7-methylguanine + DNA
-
?
additional information
-
Escherichia coli ?
-
?
additional information no activity on O2-methylcytosine, O2-methylthymine, O4-methylthymine or O6-methylguanine Micrococcus luteus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
65 min time for 50% inactivation Escherichia coli
50
-
3.5 min, 50% inactivation Micrococcus luteus
60
-
heat stable, retains 40% of its original activity after 2 min incubation Micrococcus luteus

Cofactor

Cofactor Comment Organism Structure
additional information no absolute cofactor requirement Micrococcus luteus