Cloned (Comment) | Organism |
---|---|
expression of His-tagged hARH1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant hARH1 in complex with K+ and ADP, mixing of equal volumes of protein solution containing 15-20 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl and 3 mM DTT with reservoir solution containing 150 mM lithium acetate and 25% w/v PEG 3350, at 20°C, equilibration over 0.75 ml reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | ADPRH cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P54944 | hARH1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged hARH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ADPRH cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribosylhydrolase 1 | - |
Homo sapiens |
ADPRH | - |
Homo sapiens |
hARH1 | - |
Homo sapiens |