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Literature summary for 3.2.2.19 extracted from
- ADP-Ribosylarginine hydrolases (1994), Mol. Cell. Biochem., 138, 119-122.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Mus musculus |  |
| dithiothreitol | - |
Rattus norvegicus | |
| dithiothreitol | activated by Mg2+ and dithiothreitol | Meleagris gallopavo | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a glutathione S-transferase-linked fusion protein in Escherichia coli | Rattus norvegicus |
| expression in Escherichia coli | Mus musculus |
| wild-type and mutant rat hydrolases are expressed in Escherichia coli | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition by ADP stronger than by AMP | Meleagris gallopavo | |
| ADP | - |
Rattus norvegicus | |
| ADPribose | inhibition by ADPribose stronger than by ADP and AMP | Meleagris gallopavo | |
| ADPribose | - |
Oryctolagus cuniculus | |
| ADPribose | - |
Rattus norvegicus | |
| AMP | - |
Meleagris gallopavo | |
| dithiothreitol | incubation in 20 mM at 37°C leads to rapid loss of activity but addition of Mg2+ stabilizes the hydrolase against thermal inactivation | Meleagris gallopavo | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Meleagris gallopavo | |
| Mg2+ | - |
Mus musculus | |
| Mg2+ | - |
Rattus norvegicus | |
| Mg2+ | - |
Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
- |
Mus musculus |
| 39000 | - |
- |
Rattus norvegicus |
| 39000 | - |
gel filtration, SDS-PAGE | Meleagris gallopavo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose-arginine + H2O | Meleagris gallopavo | - |
ADP-ribose + arginine | - |
? | |
| ADP-ribose-arginine + H2O | Rhodospirillum rubrum | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | ADP-ribose + arginine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meleagris gallopavo | - |
- |
- |
| Mus musculus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Rhodospirillum rubrum | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 20000 fold by DE-52, phenyl-Sepharose, hydroxylapatite, Affi-Gel 501 organo-mercurial agarose, Ultrogel AcA 54 | Rattus norvegicus |
| 200000fold by DE-52, phenyl-Sepharose, hydroxylapatite, Ultrogel AcA 54, Mono Q chromatography | Meleagris gallopavo |
| partial | Mus musculus |
| partial | Sus scrofa |
| partial | Oryctolagus cuniculus |
| partial | Ovis aries |
| partial by successive chromatography on DE-52 cellulose, phenyl-Sepharose and organomercurial agarose | Meleagris gallopavo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Meleagris gallopavo | - |
| fibroblast | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribose-arginine + H2O | - |
Meleagris gallopavo | ADP-ribose + arginine | - |
? | |
| ADP-ribose-arginine + H2O | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | Rhodospirillum rubrum | ADP-ribose + arginine | - |
? | |
| histones ADP-ribosylated + H2O | - |
Mus musculus | ? | - |
? | |
| lysozyme ADP-ribosylated + H2O | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000, SDS-PAGE | Meleagris gallopavo |
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