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Literature summary for 3.2.2.19 extracted from
- Molecular and immunological characterization of ADP-ribosylarginine hydrolases (1992), J. Biol. Chem., 267, 10481-10488.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Meleagris gallopavo |  |
| dithiothreitol | synergistically stimulated by Mg2+ and dithiothreitol | Mus musculus | |
| dithiothreitol | synergistically stimulated by Mg2+ and dithiothreitol | Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a glutathione S-transferase-linked fusion protein in Escherichia coli | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Meleagris gallopavo | |
| Mg2+ | - |
Cavia porcellus | |
| Mg2+ | - |
Sus scrofa | |
| Mg2+ | - |
Bos taurus | |
| Mg2+ | synergistically stimulated by Mg2+ and dithiothreitol | Mus musculus | |
| Mg2+ | synergistically stimulated by Mg2+ and dithiothreitol | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
SDS-PAGE | Rattus norvegicus |
| 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Meleagris gallopavo |
| 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Mus musculus |
| 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Rattus norvegicus |
| 39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Cavia porcellus | - |
- |
- |
| Meleagris gallopavo | - |
- |
- |
| Mus musculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Rhodospirillum rubrum | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 20000 fold by DE-52, phenyl-Sepharose, hydroxylapatite, Affi-Gel 501 organo-mercurial agarose, Ultrogel AcA 54 | Rattus norvegicus |
| partial | Meleagris gallopavo |
| partial | Cavia porcellus |
| partial | Mus musculus |
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cavia porcellus | - |
| brain | - |
Mus musculus | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
Sus scrofa | - |
| brain | - |
Bos taurus | - |
| brain | - |
Ovis aries | - |
| erythrocyte | - |
Meleagris gallopavo | - |
| heart | - |
Mus musculus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00008 | - |
partially purified from kidney | Rattus norvegicus |
| 0.00009 | - |
partially purified from liver | Rattus norvegicus |
| 0.00009 | - |
partially purified from muscle | Rattus norvegicus |
| 0.00016 | - |
partially purified from heart | Rattus norvegicus |
| 0.00016 | - |
partially purified from lung | Rattus norvegicus |
| 0.00045 | - |
partially purified from spleen | Rattus norvegicus |
| 0.00046 | - |
partially purified from testis | Rattus norvegicus |
| 0.00049 | - |
partially purified from brain | Rattus norvegicus |
| 3.15 | - |
after 20000fold purification | Rattus norvegicus |
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