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Literature summary for 3.2.2.17 extracted from

  • Vasquez, D.A.; Nyaga, S.G.; Lloyd, R.S.
    Purification and characterization of a novel UV lesion-specific DNA glycosylase/AP lyase from Bacillus sphaericus (2000), Mutat. Res., 459, 307-316.
    View publication on PubMed
Search for more literature for 3.2.2.17

Inhibitors

Inhibitors Comment Organism Structure
NaBH4 reduces the imino intermediate resulting in a stable covalent linkage between the enzyme and the DNA Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, SDS-PAGE and gel filtration Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeinity, using several chromatographic steps Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information unable to incise DNA containing either A:G, A:8-oxoG or 8-oxoG:C mismatches Lysinibacillus sphaericus ?
-
 ?
oligonucleotide + H2O 49-mers containing a single site-specific cis-syn, trans-syn I or trans-syn II thymine dimer Lysinibacillus sphaericus ? beta elimination reaction results in products wich have 3'4-hydroxy-2-pentanal ends ?
oligonucleotide + H2O 49-mers radiolabeled containing a site-specific uracil with uracil DNA glycosylase Lysinibacillus sphaericus ? incise of AP sites resulting in beta- and delta-elimination products with 3'4-hydroxy-2-pentenal and 3'-phosphate termini respectively ?
oligonucleotide + H2O containing either a (6-4) or Dewar photoproduct of TpT Lysinibacillus sphaericus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE and gel filtration Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
Bsp-pdg
-
 Lysinibacillus sphaericus