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Literature summary for 3.2.2.17 extracted from

  • Haseltine, W.A.; Gordon, L.K.; Lindan, C.P.; Grafstrom, R.H.; Shaper, N.L.; Gropssmann, L.
    Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus (1980), Nature, 285, 634-641.
    View publication on PubMed
Search for more literature for 3.2.2.17

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 catalytic activity 7 times greater than in presence of EDTA, DNA glycosylase activity preceded by a Mg2+ dependent AP endonuclease, followed by an AP endonuclease active in the absence of Mg2+ and in presence of EDTA Micrococcus luteus
MgCl2 not stimulated by CaCl2, MnCl2, ZnCl2 Micrococcus luteus
MgCl2 unaffected by the presence of ATP Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Micrococcus luteus pyrimidine cyclobutane dimer formed between adjacent pyrimidines ?
-
 ?
additional information Micrococcus luteus only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Reaction

Reaction Comment Organism Reaction ID
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site cleaves N-glycosyl bond between pyrimidine and sugar without rupture of a phosphodiester bond Micrococcus luteus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Micrococcus luteus thymine + ?
-
 ?
DNA + H2O pyrimidine cyclobutane dimer formed between adjacent pyrimidines Micrococcus luteus ?
-
 ?
DNA phiX174 RFI + H2O double-stranded covalently closed circular DNA molecule contains apurinic sites Micrococcus luteus thymine + ?
-
 ?
additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Micrococcus luteus ?
-
 ?

Synonyms

Synonyms Comment Organism
dimer specific M. luteus endonuclease
-
 Micrococcus luteus
dimer-dependent endonuclease
-
 Micrococcus luteus
UV endonuclease
-
 Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
 Micrococcus luteus