Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.17 extracted from

  • Nakabeppu, Y.; Sekiguchi, M.
    Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA (1981), Proc. Natl. Acad. Sci. USA, 78, 2742-2746.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.17

Application

Application Comment Organism
analysis analysis of cellular repair mechanisms Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 gel filtration Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
 T4 D, T4v1, T4uvs-5, T4uvs-13, host Escherichia coli B, Escherichia coli 1100, Escherichia coli Cr63
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Synonyms

Synonyms Comment Organism
endodeoxyribonuclease (apurinic or apyrimidinic) formerly designated EC 2.1.25.2 Tequatrovirus T4
endodeoxyribonuclease (pyrimidine dimer) formerly designated EC 3.1.25.1 Tequatrovirus T4
endonuclease V formerly designated EC 3.1.25.1 Tequatrovirus T4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 AP endonuclease destroyed very rapidly, glycosylase activity lost at linear rate of 4% per min Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 pH optimum for the 2 enzymatic activities differs, AP lyase function at pH 6.5, becoming inactive at pH 8 Tequatrovirus T4
6.5
-
 activity to form nicks, almost inactive at pH 8 or above Tequatrovirus T4

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 activity to produce alkali-labile sites Tequatrovirus T4